Dr. Heinz-Peter Nasheuer

• Career history:
  
  Since 2009 Head of School of Natural Sciences, NUI Galway
  Since 2007 Senior Lecturer in Biochemistry, School of Natural Sciences, NUI Galway.
  2003-2007 Lecturer at the Biochemistry Dept. of the Nat. University of Ireland, Galway (NUIG), Galway, Ireland as a permanent faculty member.
  March 1999 Habilitation in Biochemistry, Faculty of Biology, Friedrich Schiller University (FSU) Jena, Germany.
  1996-2002 Group leader at the Dept. of Biochemistry, Institute for Molecular Biotechnology (IMB) Jena, Germany.
  1990-1996 Group leader at the Institute of Biochemistry, University of Munich, Germany.
  1988-1990 Fellowship of the Deutsche Forschungsgemeinschaft, Stanford School of Medicine, Stanford University, CA, USA.
  1987-1988 Post-doc at the Dept. of Chemistry, Max Planck Institute for Experimental Medicine, Goettingen, Germany.
  1985-1987 Graduate student at the Dept. of Chemistry, Max Planck Institute for Experimental Medicine, Goettingen, Germany, PhD.
  1979-1984 University of Hannover (Germany), Master of Biochemistry


• Research interests:
  
  Control of DNA Replication in the Eukaryotic Cell Cycle

  The control of DNA replication and also chromosomal DNA synthesis itself are highly co-ordinated processes. To this end the replication proteins ORC (origin recognition complex), CDC6 (cell division cycle), MCM2 to MCM7 (minichromosome maintenance), CDC45, pol-prim (DNA polymerase alpha-primase), and RPA (replication protein A) must co-operate during cellular DNA replication.

  The activity of the replication and regulatory proteins is controlled by protein-protein interactions. In order to analyse the physical interactions, we have used various biochemical and cell biological techniques e.g., co-immunoprecipitation, fluorescence (cross) correlation spectroscopy (F(C)CS), a modified ELISA technique, fluorescence recovery after photobleaching (FRAP), functional test systems, and biophysical methods. In addition, we also analyse physical interactions by binding of purified proteins with radioactively labelled or fluorescence labelled interaction partners. Moreover, we have examined the functional interactions of pol-prim and RPA with kinases and phosphatases. Moreover, we have produced phosphorylation mutants of pol-prim and RPA and phosphopeptide-specific antibodies. To study their activities in living cells we expressed fusion protein of green fluorescent proteins and wild type as well as mutant proteins in human cells and have carried FCS and FRAP.

• Key publications

  Tikhanovich,I and Nasheuer, H.P. (2010) Host-Specific Replication of BK Virus DNA in Mouse Cell Extracts is Independently Controlled by DNA Polymerase{alpha}-Primase and Inhibitory Activities. J Virol, 84: 6636-6644.
  
  Fleming, R.M.T., Thiele, I. and Nasheuer H.P. (2009) Quatitative assignment of reaction directionality in constraint-based models of metabolism:Application to Esherichia coli. Biophysical Chemistry, 145: 47-56.
  
  Stephan, H., Concannon,C., Kremmer, E., Carty, M.P. and Nasheuer, H.P. (2009) Ionizing radiation-dependent and independent phosphorylation of the 32 kDa subunit of replication protein A during mitosis. Nuclecic Acids Res, 37:6028-6041.
  
  Mahon, C., Liang, B., Tikhanovich, I.S., Abend, J.R., Imperiale, M.J., Nasheuer, H.P.* and Folk, W.R.*. (2009) Restriction of human polyomavirus BKV DNA replication in murine cells and extracts, J.Virol. 83:5708-5717. * corresponding authors.
  
  Bauerschmidt, C., Pollok, S., Kremmer, E., Nasheuer, H.P.* and Grosse F.* (2007) Interactions of Human Cdc45 with the Mcm2-7 complex, the GINS complex and DNA polymerases and during S phase. Genes to Cells 12: 745-358. *corresponding authors

• Braet, C., Stephan, H., Dobbie, I. M., Togashi, D. M., Ryder, A. G., Foldes-Papp, Z., Lowndes N. and Nasheuer, H. P. (2007). Mobility and distribution of replication protein A in living cells using fluorescence correlation spectroscopy. Exp Mol Pathol. 82: 156-162.
  
  Full Publication List

• Lab members 
  
  Ronan Broderick: Postgraduate Student
  Aoife Corduff: Postgraduate Student
  Dr Mark Harvey
  Dr Siva R.K.Ramadurai
  Irina Tikhanovich

• Lab Photos
  


• Lectures/courses
  

  Course director for 3rd Biochemistry lecture course and practicals (jointly with Dr. Peter Creighton)

  Student consultation hours: Wednesday and Thursday 14:00 to 17:00

  
  Biochemistry for 2nd year Science students ’Carbohydrate Metabolism’
  Biochemistry for 3rd year Science students ’Cellular organisation and functions’ ’DNA replication’ and ’Cell signalling’
  Biochemistry lab classes for 3rd year Science students
  Biochemistry for 4th year Science students ’Cell structure, cell cycle control, and mechanisms of eukaryotic DNA replication’
  Biochemistry for 4th year Biotechnology students ’Animal tissue culture’



• Contact details
  Telephone: 353-91-49 24 30.
  Email: h.nasheuernuigalway.ie

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