Ian Dobbie

I am a Postdoc in Noel Lowndes’ Lab. Originally I trained as a physicist but I have slowly lapsed into biology. My main expertise is in microscopy, particularly in fluorescent microscopy of living cells. I am especially interested in advanced fluorescent microscopy techniques such as Fluorescence Resonance Energy Transfer (FRET, used to detect interaction between labelled molecules) and Fluorescence Correlation Spectroscopy (FCS, used to determine concentrations and diffusion rates of labelled molecules). Work in my previous lab (The Light Microscopy Group at Cancer Research UK, www.cancer.org.uk) in collaboration with Graham Dunn’s Lab at the Randall Centre, King’s College London (www.kcl.ac.uk) produced a new imaging fluorescent technique, Fluorescence Localisation After Photobleaching (FLAP) and used it to show significant new results on actin flow in motile cells ( click for the URL.).
Since joining the Lowndes lab I have been adding molecular biology and yeast culture techniques to my repertoire. At present I am working on localisations and interactions of a variety of proteins from cell cycle, DNA repair and checkpoint signalling pathways in yeast. Genomic copies of the genes will be replaced with green, yellow or cyan fluorescent fusion proteins and fluorescence microscopy used to characterise the proteins and their interactions.

When not in the lab I cycle and walk in the fantastic countryside around Galway and I surf on the Clare and Connemara coast whenever time and the waves permit.

Email: Ian.Dobbienuigalway.ie

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