Dr Brian McDonagh

BSc., MSc., PhD

Contact Details

Lecturer Below The Bar
E: BRIAN.MCDONAGH@nuigalway.ie
 
researcher

Biography

B.Sc (Hons) Biotechnology NUI Galway
M.Sc Toxicology, Athlone Institute of Technology
Hoffmann La Roche Basel, Switzerland, Research Scientist
PhD Biochemistry UCC
Fellow Higher Education Authority/ Certificate in Postgraduate Teaching University of Liverpool
Postdoctoral Experience, Universidad de Cordoba, Spain 2007-2012
Lecturer University of Liverpool 2013-2016
Lecturer NUI Galway 2017-

Research Interests

Redox signalling
Oxidative stress and reductive stress
Autophagy
Ageing
Skeletal muscle biology
Proteomics and post translational modifications
Redox relays
Peroxiredoxins, thioredoxins and glutaredoxin
microRNA's as therapeutics

Peer Reviewed Journals

  Year Publication
(2017) 'Comparison of whole body SOD1 knockout with muscle specific SOD1 knockout mice reveals a role for nerve redox signaling in regulation of degenerative pathways in skeletal muscle'
Sakellariou, G; McDonagh, B; Porter, H; Giakoumaki, I; Earl, K; Nye, G; Vasilaki, A; Brooks, S; Richardson, A; Van Remmen, H; McArdle, A; Jackson, MJ (2017) 'Comparison of whole body SOD1 knockout with muscle specific SOD1 knockout mice reveals a role for nerve redox signaling in regulation of degenerative pathways in skeletal muscle'. Antioxidants & Redox Signaling, [Details]
(2017) 'Redox Homeostasis and age-related deficits in neuromuscular integrity and function'
Sakellariou, GK; Lightfoot, SP; Earl, KE; Stofanko, M; McDonagh, B (2017) 'Redox Homeostasis and age-related deficits in neuromuscular integrity and function'. Journal of Cachexia, Sarcopenia and Muscle, [DOI] [Details]
(2017) 'Detection of ROS Induced Proteomic Signatures by Mass Spectrometry'
McDonagh, B (2017) 'Detection of ROS Induced Proteomic Signatures by Mass Spectrometry'. Frontiers in physiology, 8 [DOI] [ARAN Link] [Details]
(2016) 'Ageing-induced changes in the redox status of peripheral motor nerves imply an effect on redox signalling rather than oxidative damage'
McDonagh, B;Scullion, SM;Vasilaki, A;Pollock, N;McArdle, A;Jackson, MJ (2016) 'Ageing-induced changes in the redox status of peripheral motor nerves imply an effect on redox signalling rather than oxidative damage'. Free Radical Biology & Medicine, 94 :27-35 [DOI] [Details]
(2016) 'Ageing in relation to skeletal muscle dysfunction: redox homoeostasis to regulation of gene expression'
Goljanek-Whysall, K;Iwanejko, LA;Vasilaki, A;Pekovic-Vaughan, V;McDonagh, B (2016) 'Ageing in relation to skeletal muscle dysfunction: redox homoeostasis to regulation of gene expression'. Mammalian Genome, :341-357 [DOI] [Details]
(2016) 'Glutathione Is the Resolving Thiol for Thioredoxin Peroxidase Activity of 1-Cys Peroxiredoxin Without Being Consumed During the Catalytic Cycle'
Pedrajas, JR;McDonagh, B;Hernandez-Torres, F;Miranda-Vizuete, A;Gonzalez-Ojeda, R;Martinez-Galisteo, E;Padilla, CA;Barcena, JA (2016) 'Glutathione Is the Resolving Thiol for Thioredoxin Peroxidase Activity of 1-Cys Peroxiredoxin Without Being Consumed During the Catalytic Cycle'. Antioxidants & Redox Signaling, 24 :115-128 [DOI] [Details]
(2015) 'Redox proteomic analysis of the gastrocnemius muscle from adult and old mice'
McDonagh, B; Sakellariou, GK; Smith, NT; Brownridge, P; Jackson MJ (2015) 'Redox proteomic analysis of the gastrocnemius muscle from adult and old mice'. Data In Brief, 4 :344-348 [DOI] [Details]
(2014) 'Application of redox proteomics to skeletal muscle aging and exercise'
McDonagh, B;Sakellariou, GK;Jackson, MJ (2014) 'Application of redox proteomics to skeletal muscle aging and exercise'. Biochemical Society Transactions, 42 :965-970 [DOI] [Details]
(2014) 'Differential Cysteine Labeling and Global Label-Free Proteomics Reveals an Altered Metabolic State in Skeletal Muscle Aging'
McDonagh, B;Sakellariou, GK;Smith, NT;Brownridge, P;Jackson, MJ (2014) 'Differential Cysteine Labeling and Global Label-Free Proteomics Reveals an Altered Metabolic State in Skeletal Muscle Aging'. Journal Of Proteome Research, 13 :5008-5021 [DOI] [Details]
(2014) 'Closing a small gap in our understanding of excitation-contraction coupling: JSR90/JFP90 is Junctophilin 1'
English, KK; Sorrentino, V; Aiello, AC; Kiely, M; Kiely, PA; McDonagh, B; Mackrill JJ (2014) 'Closing a small gap in our understanding of excitation-contraction coupling: JSR90/JFP90 is Junctophilin 1'. Clinical calcium, 1 (1):1-6 [Details]
(2013) 'Thiol Redox Sensitivity of Two Key Enzymes of Heme Biosynthesis and Pentose Phosphate Pathways: Uroporphyrinogen Decarboxylase and Transketolase'
McDonagh, B;Pedrajas, JR;Padilla, CA;Barcena, JA (2013) 'Thiol Redox Sensitivity of Two Key Enzymes of Heme Biosynthesis and Pentose Phosphate Pathways: Uroporphyrinogen Decarboxylase and Transketolase'. Oxidative Medicine And Cellular Longevity, [DOI] [Details]
(2013) 'Sunlight exposure increases the phenolic content in postharvested white grapes. An evaluation of their antioxidant activity in Saccharomyces cerevisiae'
Peinado, J;de Lerma, NL;Peralbo-Molina, A;Priego-Capote, F;de Castro, C;McDonagh, B (2013) 'Sunlight exposure increases the phenolic content in postharvested white grapes. An evaluation of their antioxidant activity in Saccharomyces cerevisiae'. Journal Of Functional Foods, 5 :1566-1575 [DOI] [Details]
(2012) 'Redox Proteomics in Study of Kidney-Associated Hypertension: New Insights to Old Diseases'
Sheehan, D,Rainville, LC,Tyther, R,McDonagh, B (2012) 'Redox Proteomics in Study of Kidney-Associated Hypertension: New Insights to Old Diseases'. Antioxidants & Redox Signaling, 17 :1560-1570 [DOI] [Details]
(2012) 'Application of iTRAQ Reagents to Relatively Quantify the Reversible Redox State of Cysteine Residues'
McDonagh, B; Martínez-Acedo, P: Vázquez, J; Padilla, CA; Sheehan, D; Bárcena, JA (2012) 'Application of iTRAQ Reagents to Relatively Quantify the Reversible Redox State of Cysteine Residues'. Journal Of Proteomics, 514847 (2012):54187-54196 [DOI] [Details]
(2012) 'Nitrogen starvation induces extensive changes in the redox proteome of Prochlorococcus sp strain SS120'
McDonagh, B;Dominguez-Martin, MA;Gomez-Baena, G;Lopez-Lozano, A;Diez, J;Barcena, JA;Fernandez, JMG (2012) 'Nitrogen starvation induces extensive changes in the redox proteome of Prochlorococcus sp strain SS120'. Environmental Microbiology Reports, 4 :257-267 [DOI] [Details]
(2011) 'Biosynthetic and Iron Metabolism Is Regulated by Thiol Proteome Changes Dependent on Glutaredoxin-2 and Mitochondrial Peroxiredoxin-1 in Saccharomyces cerevisiae'
McDonagh, B;Padilla, CA;Pedrajas, JR;Barcena, JA (2011) 'Biosynthetic and Iron Metabolism Is Regulated by Thiol Proteome Changes Dependent on Glutaredoxin-2 and Mitochondrial Peroxiredoxin-1 in Saccharomyces cerevisiae'. Journal Of Biological Chemistry, 286 :15565-15576 [DOI] [Details]
(2011) 'PROTEOMICS IN INVESTIGATION OF PROTEIN NITRATION IN KIDNEY DISEASE: TECHNICAL CHALLENGES AND PERSPECTIVES FROM THE SPONTANEOUSLY HYPERTENSIVE RAT'
Tyther, R,McDonagh, B,Sheehan, D (2011) 'PROTEOMICS IN INVESTIGATION OF PROTEIN NITRATION IN KIDNEY DISEASE: TECHNICAL CHALLENGES AND PERSPECTIVES FROM THE SPONTANEOUSLY HYPERTENSIVE RAT'. Mass Spectrometry Reviews, 30 :121-141 [DOI] [Details]
(2011) 'Thiol redox proteomics identifies differential targets of cytosolic and mitochondrial glutaredoxin-2 isoforms in Saccharomyces cerevisiae. Reversible S-glutathionylation of DHBP synthase (RIB3)'
McDonagh, B;Requejo, R;Fuentes-Almagro, CA;Ogueta, S;Barcena, JA;Padilla, CA (2011) 'Thiol redox proteomics identifies differential targets of cytosolic and mitochondrial glutaredoxin-2 isoforms in Saccharomyces cerevisiae. Reversible S-glutathionylation of DHBP synthase (RIB3)'. Journal Of Proteomics, 74 :2487-2497 [DOI] [Details]
(2010) 'Structure and function of yeast glutaredoxin 2 depend on postranslational processing and are related to subcellular distribution'
Porras, P; McDonagh, B; Pedrajas, JR; Bárcena, JA; Padilla CA (2010) 'Structure and function of yeast glutaredoxin 2 depend on postranslational processing and are related to subcellular distribution'. Biochimica Et Biophysica Acta, 1804 (4):839-845 [DOI] [Details]
(2010) 'Redox proteomics'
Sheehan, D,McDonagh, B,Barcena, JA (2010) 'Redox proteomics'. Expert Review Of Proteomics, 7 :1-4 [DOI] [Details]
(2010) 'Glutaredoxin Participates in the Reduction of Peroxides by the Mitochondrial 1-CYS Peroxiredoxin in Saccharomyces cerevisiae'
Pedrajas, JR;Padilla, CA;McDonagh, B;Barcena, JA (2010) 'Glutaredoxin Participates in the Reduction of Peroxides by the Mitochondrial 1-CYS Peroxiredoxin in Saccharomyces cerevisiae'. Antioxidants & Redox Signaling, 13 :249-258 [DOI] [Details]
(2010) 'Proteomic Profiling of Perturbed Protein Sulfenation in Renal Medulla of the Spontaneously Hypertensive Rat'
Tyther, R;Ahmeda, A;Johns, E;McDonagh, B;Sheehan, D (2010) 'Proteomic Profiling of Perturbed Protein Sulfenation in Renal Medulla of the Spontaneously Hypertensive Rat'. Journal Of Proteome Research, 9 :2678-2687 [DOI] [Details]
(2010) 'Shotgun redox proteomics in sub-proteomes trapped on functionalised beads Identification of proteins targeted by oxidative stress'
Hu, WT,Tedesco, S,McDonagh, B,Sheehan, D (2010) 'Shotgun redox proteomics in sub-proteomes trapped on functionalised beads Identification of proteins targeted by oxidative stress'. Marine Environmental Research, 69 :25-27 [DOI] [Details]
(2010) 'Selection of thiol- and disulfide-containing proteins of Escherichia coli on activated thiol-Sepharose'
Hu, WT,Tedesco, S,McDonagh, B,Barcena, JA,Keane, C,Sheehan, D (2010) 'Selection of thiol- and disulfide-containing proteins of Escherichia coli on activated thiol-Sepharose'. Analytical Biochemistry, 398 :245-253 [DOI] [Details]
(2010) 'Ubiquitination and carbonylation of proteins in the clam Ruditapes decussatus, exposed to nonylphenol using redox proteomics'
Chora, S,McDonagh, B,Sheehan, D,Starita-Geribaldi, M,Romeo, M,Bebianno, MJ (2010) 'Ubiquitination and carbonylation of proteins in the clam Ruditapes decussatus, exposed to nonylphenol using redox proteomics'. Chemosphere, 81 :1212-1217 [DOI] [Details]
(2010) 'Ubiquitination and carbonylation of proteins in the clam Ruditapes decussatus, exposed to nonylphenol using redox proteomics'
Chora, S;McDonagh, B;Sheehan, D;Starita-Geribaldi, M;Romeo, M;Bebianno, MJ (2010) 'Ubiquitination and carbonylation of proteins in the clam Ruditapes decussatus, exposed to nonylphenol using redox proteomics'. Chemosphere, 81 :1212-1217 [DOI] [Details]
(2009) 'Shotgun redox proteomics identifies specifically modified cysteines in key metabolic enzymes under oxidative stress in Saccharomyces cerevisiae'
McDonagh, B; Ogueta, S; Lasarte, G; Padilla, CA; Bárcena JA (2009) 'Shotgun redox proteomics identifies specifically modified cysteines in key metabolic enzymes under oxidative stress in Saccharomyces cerevisiae'. Journal Of Proteomics, 72 (4):677-689 [DOI] [Details]
(2008) 'Ubiquitination and carbonylation as markers of oxidative-stress in Ruditapes decussatus'
Chora, S,McDonagh, B,Sheehan, D,Starita-Geribaldi, M,Romeo, M,Bebianno, MJ (2008) 'Ubiquitination and carbonylation as markers of oxidative-stress in Ruditapes decussatus'. Marine Environmental Research, 66 :95-97 [DOI] [Details]
(2008) 'Effects of oxidative stress on protein thiols and disulphides in Mytilus edulis revealed by proteomics: Actin and protein disulphide isomerase are redox targets'
McDonagh, B,Sheehan, D (2008) 'Effects of oxidative stress on protein thiols and disulphides in Mytilus edulis revealed by proteomics: Actin and protein disulphide isomerase are redox targets'. Marine Environmental Research, 66 :193-195 [DOI] [Details]
(2008) 'Oxidative stress and bivalves: a proteomic approach'
McDonagh, B; Sheehan, D (2008) 'Oxidative stress and bivalves: a proteomic approach'. Isj-Invertebrate Survival Journal, 5 :110-123 [Details]
(2007) 'Effect of oxidative stress on protein thiols in the blue mussel Mytilus edulis: Proteomic identification of target proteins'
McDonagh, B,Sheehan, D (2007) 'Effect of oxidative stress on protein thiols in the blue mussel Mytilus edulis: Proteomic identification of target proteins'. Proteomics, 7 :3395-3403 [DOI] [Details]
(2007) 'Oxidative stress in response to xenobiotics in the blue mussel Mytilus edulis L.: Evidence for variation along a natural'
Prevodnik, A,Gardestrom, J,Lilja, K,Elfwing, T,McDonagh, B,Petrovic, N,Tedengren, M,Sheehan, D,Bollner, T (2007) 'Oxidative stress in response to xenobiotics in the blue mussel Mytilus edulis L.: Evidence for variation along a natural'. Aquatic Toxicology, 82 :63-71 [DOI] [Details]
(2006) 'Redox proteomics in the mussel Mytilus edulis'
Sheehan, D,McDonagh, B,Tyther, R (2006) 'Redox proteomics in the mussel Mytilus edulis'. Marine Environmental Research, 62 :157-157 [Details]
(2006) 'Redox proteomics in the blue mussel Mytilus edulis: Carbonylation is not a pre-requisite for ubiquitination in acute free radical-mediated oxidative stress'
McDonagh, B,Sheehan, D (2006) 'Redox proteomics in the blue mussel Mytilus edulis: Carbonylation is not a pre-requisite for ubiquitination in acute free radical-mediated oxidative stress'. Aquatic Toxicology, 79 :325-333 [DOI] [Details]
(2006) 'Two-dimensional electrophoresis analysis of glutathione affinity-selected proteins from the clam Tapes semidecussatus: Evidence for tissue-specific expression of redox proteins'
Dowling, V,McDonagh, B,Cotter, EM,O'Brien, N,van Pelt, F,O'Halloran, J,Sheehan, D (2006) 'Two-dimensional electrophoresis analysis of glutathione affinity-selected proteins from the clam Tapes semidecussatus: Evidence for tissue-specific expression of redox proteins'. Comparative Biochemistry And Physiology D-Genomics & Proteomics, 1 :267-272 [DOI] [Details]
(2006) 'Redox proteomics in the mussel, Mytilus edulis'
McDonagh, B,Tyther, R,Sheehan, D (2006) 'Redox proteomics in the mussel, Mytilus edulis'. Marine Environmental Research, 62 :101-104 [DOI] [Details]
(2005) 'Carbonylation and glutathionylation of proteins in the blue mussel Mytilus edulis detected by proteomic analysis and Western blotting: Actin as a target for oxidative stress'
McDonagh, B,Tyther, R,Sheehan, D (2005) 'Carbonylation and glutathionylation of proteins in the blue mussel Mytilus edulis detected by proteomic analysis and Western blotting: Actin as a target for oxidative stress'. Aquatic Toxicology, 73 :315-326 [DOI] [Details]

Book Chapters

  Year Publication
(2012) 'Diagonal Electrophoresis for the Detection of Protein Disulfides'
McDonagh, B (2012) 'Diagonal Electrophoresis for the Detection of Protein Disulfides' In: Methods in Molecular Biology. NJ: Springer. [DOI] [Details]
(2009) 'Diagonal Electrophoresis for Detection of Protein Disulphide Bridges'
McDonagh, B (2009) 'Diagonal Electrophoresis for Detection of Protein Disulphide Bridges' In: Methods Molecular Biology. NJ: Springer. [DOI] [Details]
(2009) 'Detection of Ubiquitination in 2DE'
McDonagh, B (2009) 'Detection of Ubiquitination in 2DE' In: Methods Molecular Biology. NJ: Springer. [DOI] [Details]
(2009) 'Immunoblotting 2DE membranes'
McDonagh, B (2009) 'Immunoblotting 2DE membranes' In: Methods Molecular Biology. NA: Springer. [DOI] [Details]

Editorial

  Year Publication
(2017) microRNA-SIRT-1 interactions: key regulators of adult skeletal muscle homeostasis?.
McCormick, R,McDonagh, B,Goljanek-Whysall, K (2017) microRNA-SIRT-1 interactions: key regulators of adult skeletal muscle homeostasis?. Editorial [DOI] [Details]
(2016) Editorial: Redox Regulation in Skeletal Muscle Aging and Exercise.
McDonagh, B (2016) Editorial: Redox Regulation in Skeletal Muscle Aging and Exercise. LAUSANNE: Editorial [DOI] [Details]

Modules Taught

  Term/Year Module Title Module Code Subject / Desc
2016-2017 MD121
2016-2017 SI435
2016-2017 MD122
2016-2017 SI212
2016-2017 NU115
2016-2017 SI208
2016-2017 SI4101

External Collaborators

  Name Organisation / Institute Country Description of Collaboration
Prof Malcolm Jackson Institute of Ageing and Chronic Disease UNITED KINGDOM
We hold a number of number of grants from Medical Research Council (MRC, UK) and National Institute of Health (NIH, USA) investigating redox imbalance in skeletal muscle ageing.
Leonardo Ferreira University of Florida U.S.A.
We hold a NIH grant to investigate redox dependent changes with age and sex in Diaphragm in a chronic heart failure model.
Jose Antonio Barcena University of Cordoba SPAIN
We hold a grant to characterize the 1-Cys Peroxiredoxin Prdx6 and its role in a variety of disease states. Also investigating the effects of eNOS overexpression and subsequent changes in the redox proteome by silencing thioredoxin and glutaredoxin
Christopher Mendias University of Michigan U.S.A.
We are investigating myosteatosis in response to rotator cuff injury through metabolomics, lipidomics, proteomics and the physiological responses. We are also investigating the mdx/TR mouse as a model of muscular dystrophy.
Katarzyna Whysall University of Liverpool UNITED KINGDOM
Targeting oxidised microRNAs as potential therapeutic against sarcopaenia
Simon Tew University of Liverpool UNITED KINGDOM
MRC grant to investigate the role of the RNA binding protein HuR in musculoskeletal development and disease
Robert Cooper University of Liverpool UNITED KINGDOM
Characterisation of skeletal muscle secretome involvement in idiopathic inflammatory myopathy aetiopathology
Colin Willoughby University of Liverpool UNITED KINGDOM
Hold a fight for sight grant, PROFILING MITOCHONDRIAL DYSFUNCTION IN GLAUCOMA: THERAPEUTIC OPPORTUNITIES