Department Staff

Dr Nina L. Tuite (B.Sc., Ph.D.)
Postdoctoral Researcher

Contact Information
Molecular Diagnostics Laboratory
Department of Microbiology
National University of Ireland, Galway
University Road
Galway
Tel (Micro): 353 91 493003 (Ext 3003)
Tel (NDC): 353 91 492079 (Ext 2079)
E-mail: nina.tuitenuigalway.ie

Research Interests

My post-doctoral research is based between Dr. Tom Barry’s Lab in the Department of Microbiology, and the DNA Diagnostics Lab at the National Diagnostics Centre at NUIG. I am working as a part of a team on an R & D project developing nucleic acid diagnostic tests for clinically relevant fungal pathogens based on a proprietary platform target technology invented at NUIG.

Background

The focus of my research throughout my PhD was to investigate the molecular basis for homocysteine toxicity using Escherichia coli as a model system. I have acquired a broad range of experimental techniques and skills, especially in the areas of microbial physiology, molecular biology, genetics, and biochemistry (including proteomics).

Techniques and expertise

• Isoelectric focusing and polyacrylamide gel electrophoresis (two-dimensional gel electrophoresis)
• SDS PAGE
• Protein purification by affinity column chromatography
• Enzyme assays and kinetic analysis
• Polymerase chain reaction (PCR), Real Time PCR and, Reverse Transcriptase-PCR (RT-PCR)
• Gene cloning
restriction digests Restriction Fragment Length Polymorphism (RFLP) analysis
• DNA Sequencing analysis, genetic transduction
• I also have a lot of experience in bacterial physiology.

Publications

• Tuite, N. L., K. R. Fraser, and P. C. O'Byrne. 2005. Homocysteine toxicity in Escherichia coli is caused by a perturbation of branched-chain amino acid biosynthesis. J. Bacteriol. 187:4362-4371.


• Fraser, K. R., N. L. Tuite, A. Bhagwat, and C. P. O'Byrne. 2006. Global effects of homocysteine on transcription in Escherichia coli: induction of the gene for the major cold-shock protein, CspA. Microbiology 152:2221-2231

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