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The following lists provide details of the presentations of research data -derived from the RDG, Department of Microbiology, NUl, Galway, Ireland.

Recombinant DNA Group - Publications

Powell, R., Neilan, J. & Gannon, F. (1986) Plaque dot assay. Nucleic Acids Research, 14:1541.

Powell, R. & Gannon, F. (1988) The Leghemoglobins. BioEssays, 9:117-121.

Gannon, F., Neilan, J. & Powell, R. (1988) Too many books spoil the cook. Nature333:309-310.

Heery, D.M., Powell, R., Gannon, F. & Dunican, L.K.  (1989) Curing of a plasmid from E.coli using high-voltage electroporation. Nucleic Acids Research, 17:10131.

Powell, R., Heery, D.M., Dawson, M.T., Dunican, L.K. & Gannon, F. (1990) Skimmed milk as an alternative to X-gal for visual discrimination of background in lambda gt10 libraries. Nucleic Acids Research, 18:2190.

Barry, T., Powell, R. & Gannon F. (1990) A general method for the development of DNA probes for micro-organisms. Nature Biotechnology, 8:233-236.

Heery, D.M., Gannon, F. & Powell, R. (1990) A simple method to isolate DNA fragments for subcloning. Trends in Genetics, 6:173

Powell, R., Heery, D.M., Gannon, F. & Dunican L.K. (1990) Electrotransfection of E. coli with lambda gt10 DNA. FEMS Microbiology Letters, 72:225-228. 

Powell, R., Wolff, J., Stack, M., Byrnes, L., Higgins, D.G., Sharp, P.M. & Gannon F. (1990) The salmon gene for apolipoprotein A-1: cDNA sequence, tissue expression and evolution. Gene, 104:155-161.   

Powell, R., Byrnes, L. & Gannon F. (1990) Sequence of a cDNA clone encoding an Atlantic salmon ribosomal protein. Gene, 101:303-304.

Powell, R., Byrnes, L. & Gannon F. (1990) Transgenic fish. The Genetic Engineer and Biotechnologist, 1:7-9.

Gannon, F., Powell, R., Barry, T., McEvoy, T.G. & Sreenan, J.M. (1990) Transgenic farm animals. Journal of Biotechnology, 16:155-170.

Colleran, G.J., Barry, T., Powell, R., Gannon, F. & Dunican, L.K. (1990) Rapid analysis of an aberrant region of the ribosomal operon from a selection of clostridial species using PCR: A new taxonomic approach. In: Proceedings of the 5th European Congress on Biotechnology, Copenhagen, Denmark. July 8th-13th. Vol. I, pp410-413.

Powell, R., Byrnes, L. & Gannon, F. (1990) Liver gene expression: Cloning of abundant mRNA transcripts from the liver of Atlantic salmon (Salmo salar), identification of the salmon apolipoprotein A-I gene. In: Proceedings of the 5th European Congress on Biotechnology, Copenhagen, Denmark. July 8th-13th. Vol. I, pp453-456.

Gannon, F. & Powell, R. (1991) Construction of recombinant DNA molecules. In: Essential Molecular Biology: A Practical Approach (ed. T.A. Brown), 1st Edition,  Vol.1, Ch. 9, pp143-160, IRL Press (at Oxford University Press), Oxford, U.K.

Horan, R., Powell, R., McQuaid, S., Gannon, F. & Houghton, J.A. (1991) Association of foreign DNA with porcine spermatazoa. Archives of Andrology, 26:83-92.

Powell, R., Byrnes, L., Barry, T. & Gannon, F. (1991) A rapid procedure to isolate major abundance mRNA transcripts and study the transcription profile of a tissue. Technique, 2:213-215.

Horan, R., Powell, R., Bird, J.M., Gannon, F. & Houghton, J.A. (1992) Effects of electropermeabilisation on the association of foreign DNA with pig sperm. Archives of Andrology, 28:105-114.

Horan, R., Powell, R., Gannon, F. & Houghton, J.A. (1992) The fate of foreign DNA associated with pig sperm following the in vitro fertilisation of zona-free hamster ova and zona-intact pig ova. Archives of Andrology, 29:199-206.

Bird, J.M., Powell, R., Horan, R., Gannon, F. & Houghton, J.A. (1992) The binding of exogenous DNA fragments to bovine spermatozoa. Animal Biotechnology, 3:181-200.

Bearzotti, M., Perrot, E., Michard-Vanhee, C., Jolivet, G., Attal, J., Theron, M.-C., Puissant, C., Dreano, M., Kopchick, J.J., Powell, R., Gannon, F., Houdebine, L.-M. & Chourrout, D. (1992) Gene expression following transfection of fish cells. Journal of Biotechnology26:315-326.

Hiney, M., Dawson, M.T., Heery, D.M., Smith, P.R., Gannon, F. & Powell, R. (1992) DNA probe for Aeromonas salmonicida. Applied & Environmental Microbiology, 58:1039-1042.

Barry, T., Geary, S., Hanniffy, S., MacGearailt, C., Shalloo, M., Heery, D.M., Gannon, F. & Powell, R. (1992) Rapid mini-preparations of total RNA from bacteria. Nucleic Acids Research, 20:4940.

Hanley, S. & Powell, R. (1994) Sequence of a cDNA clone encoding the Atlantic salmon a-1-microglobulin and bikunin Proteins. Gene, 147:297-298.

O'Brien, D., Mooney, J., Ryan, D., Powell, E., Hiney, M., Smith, P.R. & Powell, R. (1994) Detection of Aeromonas salmonicida, causal agent of furunculosis, from the effluent of hatchery-reared Atlantic salmon smolts. Applied & Environmental Microbiology, 60:3874-3877.

Mooney, J., Powell, E., Clabby, C. & Powell, R. (1995) Detection of Aeromonas salmonicida in wild Atlantic salmon using a specific DNA probe test. Diseases of Aquatic Organisms, 21:131-135.

McInerney, J.O., Paskins, L., Eardly, D., Patching, J.W. & Powell, R. (1995) Extraction of procaryotic genomic DNA from marine microbial communities suitable for amplification using the polymerase chain reaction. Internationale Revue der Gesamten Hydrobiologie, 80(2):351-360.

McInerney, J.O., Wilkinson, M., Patching, J.W., Embley, T.M. & Powell, R. (1995) Recovery and phylogenetic analysis of novel archaeal ribosomal RNA sequences from a deep-sea deposit feeder. Applied & Environmental Microbiology, 61:1646-1648.

Hanley, S., McInerney, J.O. & Powell, R. (1995) Sequence analysis and evolutionary aspects of piscine a-1-microglobulin/bikunin mRNA transcripts. Molecular Marine Biology & Biotechnology, 4(2):105-109.

Doyle, L.M., McInerney, J.O., Mooney, J., Powell, R., Haikara, A. & Moran, A.P. (1995) Sequence of the gene encoding the 16S rRNA of the beer spoilage organism Megasphaera cerevisiae. Journal of Industrial Microbiology, 15:67-70.

Conti, F.G., Powell, R., Pozzi, L., Zezze, G., Faraggiana, T., Gannon, F. & Fabbrini, A. (1995) A novel line of transgenic mice (RSV/LTR-bGH) expressing growth hormone in cardiac and striated muscle. Growth Regulation, 5:101-108.

McCormack, G., Keegan, B., McInerney, J.O. & Powell, R. (1995) Comparison of DNA extraction techniques from amphiurid species (Ophiuroidea: Echinodermata) suitable for polymerase chain reaction. In: Echinoderm Research 1995. (Ed.s R.H. Emson, A.B. Smith and A.C. Campbell). A.A. Balkema, Rotterdam. pp.43-50.

Powell, R., Mullarkey, M., O'Brien, D. & McInerney J.O. (1995) Detection of microorganisms in the environment. Biochemical Society Transactions, 23:435-437.

Dawson, M.T., Powell, R. & Gannon, F. (1996) Gene technology. The Introduction to Biotechniques Series (ed. J.M. Graham & D. Billington). 118 pages. BIOS Scientific Publishers Ltd., Oxford, U.K.

Clabby, C., Goswami, U., Flavin, F., Wilkins, N.P., Houghton, J.A. & Powell, R. (1996) Cloning, characterisation and chromosomal location of a satellite DNA from the Pacific oyster, Crassostrea gigas. Gene, 168:205-209.

Sanchez, J.A., Clabby, C., Ramos, D., Blanco, G., Vazquez, E. & Powell, R. (1996) Protein and microsatellite single locus variability in Atlantic salmon (Salmo salar L.). Heredity, 77:423-432.

Abuin, M., Clabby, C., Martinez, P., Goswami, U., Flavin, F., Wilkins, N.P., Houghton, J.A., Powell, R. & Sanchez, L. (1996) A NOR-associated repetitive element present in the genome of two Salmo Species (Salmo salar and Salmo trutta). Genome, 39:671-679.

Dineen, T.M., Powell, R. & Houghton, J.A. (1996) Mapping the Xp22.1 - Xp22.3 region of the human X chromosome by fluorescent in situ hybridization. Irish Journal of Medical Science, 165:312.

Dineen, T.M., Powell, R. & Houghton, J.A. (1996) Physical mapping in the Xp22.1-22.3 region of the human X chromosome. Journal of Medical Genetics, 33:S47.

Dineen, T.M., Powell, R. & Houghton, J.A. (1997) Application of FISH to the physical mapping of Xp22.1 - Xp22.3. Cytogenetics and Cell Genetics, 77:27.

Dineen, T.M., Powell, R. & Houghton, J.A. (1997) Gene mapping by fluorescence in situ hybridisation. Irish Journal of Medical Science, 166:177.

Dineen, T.M., Powell, R. & Houghton, J.A. (1997) Physically mapping the hypophosphataemic rickets gene on Xp22.1-22.3. Journal of Medical Genetics, 34:S70.

McInerney, J.O., Mullarkey, M., Wernecke, M.E. & Powell, R. (1997) Phylogenetic analysis of marine archaeal rRNA sequences emphasizes the hidden diversity within the primary group Archaea. Proceedings of the Royal Society, London, B., 264:1663-1669.

Austin, B., Austin, D.A., Dalsgaard, I., Gudmundsdottir, B.K., Hoie, S., Thornton, J.M., Larsen, J.L., O'hIci, B. & Powell, R. (1998) Characterization of atypical Aeromonas salmonicida by different methods. Systematic & Applied Microbiology, 21:50-64.

Powell, R., Larsen, J.L., Dalsgaard, I., Hoie, S., Gudmundsdottir, B. & Austin, B. (1998) Improved identification and taxonomy of atypical isolates of the fish pathogen Aeromonas salmonicida. In: Proceedings of the 3rd European Marine Science and Technology Conferance, Vol. I, p110-113, Lisbon, Portugal. May 23rd-27th.

Thornton, J.M., Austin, D.A., Austin, B. & Powell, R. (1999) Small subunit rRNA gene sequences of Aeromonas salmonicida subsp. smithia and Haemophilus piscium reveal pronounced similarities with A. salmonicida subsp. salmonicida. Diseases of Aquatic Organisms, 35:155-158.

Reilly, A., Elliot, N. G., Grewe, P. M., Clabby, C., Powell, R. & Ward, R.D. (1999) Genetic differentiation between Tasmanian cultured Atlantic salmon (Salmo salar L.) and their ancestral Canadian population: Comparison of microsatellite DNA and allozyme and mitochondrial DNA variation. Aquaculture, 173:459-469.

Houghton, J.A., Dineen, T.M., Speleman, F & Powell, R. (1999) Physical gene mapping using fibre FISH of sperm. Cytogenetics and Cell Genetics, 85:30.

Hoie, S., Dalsgaard, I., Aase, I.L., Heum, M., Thornton, J.M. & Powell, R. (1999) Polymerase chain reaction (PCR)-based typing of atypical isolates of the fish pathogen Aeromonas salmonicida. Systematic & Applied Microbiology, 22:403-411.

O'hIci, B., Olivier, G. & Powell, R. (2000) Genetic diversity of the fish pathogen Aeromonas salmonicida demonstrated by random amplified polymorphic DNA and pulsed-field gel electrophoresis analyses. Diseases of Aquatic Organisms, 39:109-119.

McCormack, G.P., Powell, R. & Keegan, B.F. (2000) Comparative analysis of two populations of the brittle star Amphiura filiformis (Echinodermata: Ophiuroidea) with different life style strategies using RAPD markers. Marine Biotechnology, 2:100-106.

McCormack, G.P., Keegan, B.F., McInerney, J.O. & Powell, R. (2000) Spectral analysis of echinoderm small subunit ribosomal RNA gene sequence data. Molecular Phylogenetics & Evolution, 15:327-329.

Nolan, D., Martin, S., Kelly, Y., Glennon, K., Palmer, R., Smith, T., McCormack, G.P. & Powell, R. (2000) Development of microsatellite PCR typing methodology for the sea louse, Lepeophtheirus salmonis. Aquaculture Research, 31:1-8.

McInerney, J.O., Mullarkey, M., Wernecke, M. & Powell, R. (2000) The impact of taxonomic gene libraries on our knowledge of bacterial diversity: An example using the marine Archaea. In: Biological Collections and Biodiversity (ed. B.S. Rushton, P. Hackney & C.R. Tyrie), Ch. 17, pp155-163.

Powell, R. (2000) Biotechnology in aquaculture. In: Biological Resource Management: Connecting Science and Policy. (ed. E. Balazs, E. Galante, J.M. Lynch, J.S. Schepers, J.-P. Toutant, D. Werner & P.A.Th.J. Werry), Ch. 13, pp173-186, Springer-Verlag, Berlin, Germany.

Powell, R. & Gannon, F. (2000) Construction of recombinant DNA  molecules.   In: Essential Molecular Biology: A Practical Approach (ed. T.A. Brown), 2nd Edition,  Vol. 1, Ch. 7, pp129-150, Oxford University Press, Oxford, U.K.

Davey, G.C., Caplice, N.C., Martin, S.A. & Powell, R. (2001) A survey of genes in the Atlantic salmon (Salmo salar) as identified by expressed sequence tags. Gene, 263:121-130.



Department of Microbiology, National University of Ireland, Galway.