4th year Spectroscopy Lecture Material: Alan Ryder
Course outline (2011-2012)
Reading lists
Internet Resources:
Problem Sheet
Spectroscopy (CH407: CP-406): Course Outline:
This course gives a more detailed insight into some aspects of Spectroscopy. The specific topics to be covered include:
- Excited States: What governs the absorption of radiation and what happens in the excited states.
Fluorescence Spectroscopy: A detailed look at emission from excited states, and some advanced methods.
Fluorophores: A short overview of what types of molecules and materials fluorescence.
Reading Lists:
The recommended textbooks are Physical Chemistry, Atkins & de Paula, 8th edition and Principles of Fluorescence Spectroscopy, Lakowicz, 3rd edition. Both of these are available in the library. I will also provide handouts at the lectures.
The reading is broken down into level 1(introductory & 2Y/3Y revision), level 2(core course), and level 3 (advanced).
The recommended textbooks are Physical Chemistry, Atkins & de Paula, 8th edition and Principles of Fluorescence Spectroscopy, Lakowicz, 3rd edition. Both of these are available in the library. I will also provide handouts at the lectures.
- L1 will give you a good introductory background to the various concepts covered in 4th year.
- L2 texts will give you the necessary detail and tools to be able to understand and answer the questions.
- L3 texts are for specific examples, project work, and for the essay questions.
Level 1 (Introductory & revision):
Elements of Physical Chemistry, Atkins & de Paula, 4th Edition: Chapter 20: Electronic transitions and photochemistry.
This is a basic introduction to spectroscopy. But it does not cover any of the topics in sufficient detail.
Revise 2y spectroscopy notes.
Level 2 (Core course):
The course is based on the course outline and lecture notes.
Atkins does not cover in detail all the topics which we will cover, so there are a list of additional texts that should be consulted. Some reviews are also included for some of the specific topics to help.
Physical Chemistry, Atkins & de Paula, 8th Edition (recommended textbook). Chapters 14: electronic transitions/excited states. Chapter 23: 23.7-23.8: Photochemistry/Fluorescence.
Principles of Fluorescence Spectroscopy, J.R. Lakowicz (2nd or 3rd edition): Elements of Chapters 1, 3, 6, 8.
Fluorescence Polarization/Anisotropy in Diagnostics and Imaging. D.M. Jameson and J.A. Ross, Chem. Rev. 2010, 110, 2685-2708. doi:
10.1021/cr900267p
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Internet Resources:
The following websites have useful online demos and graphics for illustrating many of the concepts covered in the lectures.
For UV-VIS spectroscopy:
For Fluorescence spectroscopy:
Molecular Probes (now owned by Invitrogen): This manufacturer of fluorescent dyes has a very comprehensive website with a lot of resources useful for understanding fluorescence techniques. The
Handbook
covers most of what we will cover.
The handbook covers a lot of the basics of fluorescence and applications in life sciences. The introduction section covers a lot of what you need to know.
The spectral viewer is very useful for selecting fluorescent probes.
You are strongly encouraged to view the two
tutorials on fluorescence from Invitrogen.
Rutgers have brief notes on most of what we will do.
Fluorescence Lifetimes:
ISS tech note and data: This company make lifetime spectrometers and microscopes. There are 2 in the NBL labs.
Fluorescence Lifetime Imaging Microscopy (FLIM) introduction from Olympus resource centre.
Fluorescence Anisotropy:
This
link has a brief review (5 pages) article on the method.
Nice intro and examples at
Rochester.
Technical note from JY-Horiba.
Fluorescence Correlation Spectroscopy (FCS):
FCS expert: a series of tutorials and examples covering FCS (very good company website).
I'll add more later.
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Leagan Gaeilge
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