- Our People
- Chemistry
Dr Peter Crowley B.Sc.,Ph.D.
Contact Details
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Biography:
| After graduating with first class honours in chemistry Peter joined
the laboratory of Prof. G. W. Canters at Leiden University, The
Netherlands. Working with his advisor Prof. M. Ubbink, he used NMR
spectroscopy to study transient protein interactions involved in
electron transport. This research was summarized in an invited paper in
Accounts of Chemical Research. From the Netherlands Peter headed south for sunnier climes. With a Marie Curie Postdoctoral fellowship he joined the lab of Prof. M. A. Carrondo at Universidade Nova de Lisboa, Portugal. During this time he further developed his interest in molecular recognition. In particular, he demonstrated the importance of cation-pi interactions in protein interfaces. In 2006, Peter returned to Ireland, working as a lecturer in Biochemistry at UCD, and since 2008 as a lecturer in Chemistry at NUI Galway. Current interests include protein self assembly and interactions under physiological conditions. For more information see publications below. |
Research Interests
| - Molecular Recognition
- Protein-protein & protein-small molecule interactions - NMR spectroscopy / X-ray crystallography / Small angle X-ray scattering - In-cell NMR spectroscopy |
Peer Reviewed Journals
| Crowley PB, Kyne C, Monteith WB. (2012) 'Simple and inexpensive incorporation of (19)F-Tryptophan for protein NMR spectroscopy'. Chemical Communications (Cambridge, England), . [Details] |
| McGovern, RE,Fernandes, H,Khan, AR,Power, NP,Crowley, PB (2012) 'Protein camouflage in cytochrome c-calixarene complexes'. Nature Chemistry, 4 :527-533. [DOI] [Details] |
| Crowley,P.B.,Chow, E.,Papkovskaia, T. (2011) 'Protein Interactions in the Escherichia coli Cytosol: An Impediment to In-Cell NMR Spectroscopy'. ChemBioChem, 12 (7):1043-1048. [DOI] [Details] |
| Farrell,D., Sá-Mira,E., Webb,H, Georgi, N., Crowley,P.B., McIntosh,L.P., Nielsen,J.E. (2010) 'Titration_DB: Storage analysis of NMR-monitored protein pH titration curves'. Proteins: Structure, Function And Bioformatics, 78 :843-857. [Details] |
| Crowley,P.B., Matias,P.M., A. R. Khan, M. Roessle, D. I. Svergun (2009) 'Metal-mediated self-assembly of a beta-sandwich protein'. Chem. Eur. J, 15 :12672-12680. [Details] |
| Yanagisawa, S.,Dennison, C.,Crowley, P.B.,Firbank, S.J.,Lawler, A.T.,Hunter, D.M.,McFarlane, W.,Li, C.,Kohzuma, T.,Banfield, M.J. (2008) 'Interaction tuning of the active site properties of metalloproteins'. Journal of the American Chemical Society, 130 (46). [Details] |
| Crowley,P.B.,Ganji, P.,Ibrahim, H. (2008) 'Protein surface recognition: Structural characterisation of cytochrome c-porphyrin complexes'. ChemBioChem, 9 (7). [Details] |
| Petoukhov, M.V.,Vicente, J.B.,Crowley, P.B.,Carrondo, M.A.,Teixeira, M.,Svergun, D.I. (2008) 'Quaternary Structure of Flavorubredoxin as Revealed by Synchrotron Radiation Small-Angle X-Ray Scattering'. Structure, 16 (9). [Details] |
| Crowley, P.B.,Matias, P.M.,Mi, H.,Firbank, S.J.,Banfield, M.J.,Dennison, C. (2008) 'Regulation of protein function: Crystal packing interfaces and conformational dimerization'. Biochemistry, 47 (25). [Details] |
| Crowley,P.B.,Brett, K.,Muldoon, J. (2008) 'NMR spectroscopy revceals cytochrome c-poly(ethylene glycol) interactions'. ChemBioChem, 9 (5). [Details] |
| Sato,K.,Crowley,P.B.,Dennison,C. (2007) 'Transient protein interactions of cytochrome f and the phytocyanin umecyanin'. ChemBioChem, 8 :732-735. [Details] |
| Crowley,P.B.,Golovin,A. (2005) 'Cation-pi interactions in protein-protein interfaces'. Proteins: Structure, Function And Bioformatics, 59 :231-239. [Details] |
| Sato, K.,Crowley, P.B.,Dennison, C. (2005) 'Transient homodimer interactions studied using the electron self-exchange reaction'. Journal of Biological Chemistry, 280 (19). [Details] |
| Diaz-Moreno,I.,Diaz-Quintana,A.,De La Rosa, M.A.,Crowley,P.B.,Ubbink, M. (2005) 'Different modes of interaction in cyanobacterial complexes of plastocyanin and cytochrome f'. Biochemistry, 44 (9). [Details] |
| Crowley,P.B.,Carrondo, M.A. (2004) 'The Architecture of the Binding Site in Redox Protein Complexes: Implications for Fast Dissociation'. Proteins: Structure, Function and Genetics, 55 (3). [Details] |
| Crowley, P.B.,Hunter, D.M.,Sato, K.,McFarlane, W.,Dennison, C. (2004) 'The parsley plastocyanin-turnip cytochrome f complex: A structurally distorted but kinetically functional acidic patch'. Biochemical Journal, 378 (1). [Details] |
| Crowley, P.B.,Ubbink, M. (2003) 'Close Encounters of the Transient Kind: Protein Interactions in the Photosynthetic Redox Chain Investigated by NMR Spectroscopy'. Accounts of Chemical Research, 36 (10). [Details] |
| Dunne,E.C.,Coyne,E.J.,Crowley,P.B.,Gilheany,D.G. (2002) 'Co-operative ortho-effects on the Wittig reaction Interpretation of stereoselectivity in the reaction of ortho-halo-substituted benzaldehydes and benzylidenetriphenyl-phosphoranes'. Tetrahedron Lett, 43 :2449-2453. [Details] |
| Crowley, P.B.,Rabe, K.S.,Worrall, J.A.R.,Canters, G.W.,Ubbink, M. (2002) 'The ternary complex of cytochrome f and cytochrome c: Identification of a second binding site and competition for plastocyanin binding'. ChemBioChem, 3 (6). [Details] |
| Worrall, J.A.R.,Liu, Y.,Crowley, P.B.,Nocek, J.M.,Hoffman, B.M.,Ubbink, M. (2002) 'Myoglobin and cytochrome b5: A nuclear magnetic resonance study of a highly dynamic protein complex'. Biochemistry, 41 (39). [Details] |
| Crowley, P.B.,Diaz-Quintana, A.,Molina-Heredia, F.P.,Nieto, P.,Sutter, M.,Haehnel, W.,De la Rosa, M.A.,Ubbink, M. (2002) 'The interactions of cyanobacterial cytochrome c6 and cytochrome f, characterized by NMR'. Journal of Biological Chemistry, 277 (50). [Details] |
| Crowley,P.B.,Vintonenko, N.,Bullerjahn,G.S,Ubbink,M. (2002) 'Plastocyanin-cytochrome f interactions: The influence of hydrophobic patch mutations studied by NMR spectroscopy'. Biochemistry, 41 :15698-15705. [Details] |
| Crowley,P.B.,Otting, G.,Schlarb-Ridley, B.G.,Canters, G.W.,Ubbink, M. (2001) 'Hydrophobic interactions in a cyanobacterial plastocyanin - Cytochrome f complex'. Journal of the American Chemical Society, 123 (43). [Details] |
| Crowley, P.,Ubbink,M.,Otting.G. (2000) 'Angle restraints in protein backbones from dipole-dipole cross-correlation between 1HN-15N and 1HN-1H vectors'. Journal Of The American Chemical Society, 122 :2968-2969. [Details] |
Teaching Interests
| Peter teaches on various lecture and practical courses in the School of Chemistry. The two main contributions are: CH431 - 12 lectures on protein interactions and structural biology CH311 - 9 lectures on protein structure and natural products |
Recent Postgraduates
| PhD Students:
Roise Mc Govern BSc, funded by NUI Galway college fellowship - Protein-small molecule interactions studied by NMR and X-ray crystallography Giada Cattani MSc, funded by Science Foundation Ireland - Controlled protein self assembly using thiol conjugates Ciara Kyne BSc, funded by NUI Hardiman Fellowship and NUI Travelling Studentship - In-cell NMR studies of GB1 Pawel Antonik MSc, funded by Teagasc Walsh Fellowship and NUI Galway Millennium Fund - Structural characterisation of a fucose-binding lectin Madeleine Mallon BSc, funded by NUI Hardiman Fellowship - Protein-ligand interactions studied by X-ray crystallography |
Internal Collaborators
| Dr. Andrew Flaus Prof. Kevin Sullivan |
External Collaborators
| Prof. Christopher Dennison (Newcastle University)
Prof. Jan van Hest (Radboud University Nijmegen) Dr. Amir Khan (Trinity College Dublin) Dr. Jens Nielsen (University College Dublin) Prof. Gary Pielak (University of North Carolina at Chapel Hill) Dr. Dmitri Svergun (EMBL, Hamburg) |