Research Interests

cancer biology, cell cycle control, DNA replication, initiation, DNA repair signalling, protein biochemistry, cell biology, fluorescence correlation spectrocopy, protein-DNA and protein-protein interaction, microscopy

 

Books

Nasheuer, HP (2010) Genome stability & human diseases. Berlin, Germany: Springer. [Details]

Book Chapters

Conmy, S., and Nasheuer H.P. (2010) 'The use of transgenic mice in cancer and genome stability research' In: Nasheuer, H.P (eds). Genome stability and human diseases. Berlin: Springer. [Details]

Broderick, S, Rehmet, K., Concannon, C., and Nasheuer H.P. (2010) 'Eukaryotic single-stranded DNA binding proteins: Central factors in genome stability' In: Nasheuer, H.P (eds). Genome stability and human diseases. Berlin: Springer. [Details]

Pisani, F. M., De Felice, M., De Falco, M., Pollok, S. and Nasheuer, H.P. (2008) 'Regulation of Eukaryotic DNA Replication at the Initiation Step' In: Maga, G (eds). DNA Replication, Repair and Recombination at the Crossroad Between Genome Integrity and Genomic Instability. Trivandrum, Indien: Research Signpost, Trans-world Research Network. [Details]

Nasheuer, H.P., Pospiech, H., and Syväoja, J. (2006) 'Progress towards the anatomy of the eukaryotic DNA replication fork' In: Lankenau, DH (eds). Genome Integrity: Facets and Perspectives. Berlin, Germany: Springer. [Details]

Nasheuer, H.P., Pospiech, H., and Syväoja, J. (2006) 'Human DNA Polymerases' In: D. Ganten and K. Ruckpaul (eds). Encyclopedic Reference of Genomics and Proteomics in Molecular Medicine. Berlin Heidelberg, Germany: Springer. [Details]

Smith, R. W. P., and Nasheuer, H.P. (2000) 'DNA replication systems in vivo and in vitro as tools to study virus host interactions' In: Pandalai (eds). Recent Research Developments in Virology. Trivandrum, India: Transworld Research Network. [Details]

Schneider, C., Winkler, D. v., Dornreiter, I., Nasheuer, H.P., Gilbert, I., Dehde, S., Arthur, A., and Fanning, E. (1992) 'Bovine RBovine RP-A functions in SV40 DNA replication in vitro, but bovine DNA polymerase α-primase inhibits replication completely' In: M. Kohiyama & P. Hughes (eds). Regulatory Mechanisms of DNA Replication. Berlin, Germany: Springer. [Details]

Grosse, F. and Nasheuer, H.P. (1988) 'Structure and properties of the immunoaffinity-purified DNA polymerase α-primase complex' In: B. Stillman & T. Kelly (eds). Cancer Cells Vol. 6, Eukaryotic DNA Replication. Cold Spring Harbor, New York, USA: Cold Spring Harbor Press. [Details]

 

Peer Reviewed Journals

Liang, B,Tikhanovich, I,Nasheuer, HP,Folk, WR (2012) 'Stimulation of BK Virus DNA Replication by NFI Family Transcription Factors'. J Virol, 86 :3264-3275. [DOI] [Details]

Broderick, R,Ramadurai, S,Toth, K,Togashi, DM,Ryder, AG,Langowski, J,Nasheuer, HP (2012) 'Cell Cycle-Dependent Mobility of Cdc45 Determined in vivo by Fluorescence Correlation Spectroscopy'. Plos One, 7 . [DOI] [Details]

Tikhanovich I, Liang B, Seoighe C, Folk WR, Nasheuer HP. (2011) 'Inhibition of Human BK Polyomavirus Replication by Small Noncoding RNAs'. J Virol, 85 (14):6930-6940. [Details]

Zhang, SS,Roche, K,Nasheuer, HP,Lowndes, NF (2011) 'Modification of Histones by Sugar beta-N-Acetylglucosamine (GlcNAc) Occurs on Multiple Residues, Including Histone H3 Serine 10, and Is Cell Cycle-regulated'. Journal Of Biological Chemistry, 286 :37483-37495. [DOI] [Details]

Tikhanovich, I,Nasheuer, HP (2010) 'Host-Specific Replication of BK Virus DNA in Mouse Cell Extracts Is Independently Controlled by DNA Polymerase alpha-Primase and Inhibitory Activities'. J Virol, 84 :6636-6644. [DOI] [Details]

Fleming, RMT,Thiele, I,Provan, G,Nasheuer, HP (2010) 'Integrated stoichiometric, thermodynamic and kinetic modelling of steady state metabolism'. Journal Of Theoretical Biology, 264 :683-692. [DOI] [Details]

Stephan, H,Concannon, C,Kremmer, E,Carty, MP,Nasheuer, HP (2009) 'Ionizing radiation-dependent and independent phosphorylation of the 32-kDa subunit of replication protein A during mitosis'. Nucleic Acids Research, 37 :6028-6041. [DOI] [Details]

Fleming, RMT,Thiele, I,Nasheuer, HP (2009) 'Quantitative assignment of reaction directionality in constraint-based models of metabolism: Application to Escherichia coli'. Biophysical Chemistry, 145 :47-56. [DOI] [Details]

Levy, N,Oehlmann, M,Delalande, F,Nasheuer, HP,Van Dorsselaer, A,Schreiber, V,de Murcia, G,Menissier-de Murcia, J,Maiorano, D,Bresson, A (2009) 'XRCC1 interacts with the p58 subunit of DNA Pol -primase and may coordinate DNA repair and replication during S phase'. Nucleic Acids Research, 37 :3177-3188. [DOI] [Details]

Mahon, C., Liang, B., Tikhanovich, I., Abend, J.R., Imperiale, M.J., Nasheuer, H.P.* and Folk, W.R.* (2009) 'Restriction of human polyomavirus BKV DNA replication in murine cells and extracts'. J Virol, 83 (11):5708-5717. [ARAN Link] [Details]

Flemming, R.M.T. and Nasheuer, H.P. (2009) 'Practical issues in the detection of multiscale spatial synchronization using empirical mode decomposition'. Advances in Adaptive Data Analysis, . [Details]

Flemming, R.M.T. and Nasheuer, H.P. (2008) 'Practical issues in the detection of multiscale spatial synchronization using empirical mode decomposition'. Advances in Adaptive Data Analysis, . [Details]

Taneja, P,Boche, I,Hartmann, H,Nasheuer, HP,Grosse, F,Fanning, E,Weisshart, K (2007) 'Different activities of the largest subunit of replication protein A cooperate during SV40 DNA replication'. Febs Letters, 581 :3973-3978. [DOI] [Details]

Braet, C,Stephan, H,Dobbie, IM,Togashi, DM,Ryder, AG,Foldes-Papp, Z,Lowndes, N,Nasheuer, HP (2007) 'Mobility and distribution of replication protein A in living cells using fluorescence correlation spectroscopy'. Experimental And Molecular Pathology, 82 :156-162. [DOI] [Details]

Taneja, P,Nasheuer, HP,Hartmann, H,Grosse, F,Fanning, E,Weisshart, K (2007) 'Timed interactions between viral and cellular replication factors during the initiation of SV40 in vitro DNA replication'. Biochemical Journal, 407 :313-320. [DOI] [Details]

Bauerschmidt, C,Pollok, S,Kremmer, E,Nasheuer, HP,Grosse, F (2007) 'Interactions of human Cdc45 with the Mcm2-7 complex, the GINS complex, and DNA polymerases delta and epsilon during S phase'. Genes To Cells, 12 :745-758. [DOI] [Details]

Pollok, S,Bauerschmidt, C,Sanger, J,Nasheuer, HP,Grosse, F (2007) 'Human Cdc45 is a proliferation-associated antigen'. Febs Journal, 274 :3669-3684. [DOI] [Details]

Rytkönen, A. K., Vaara, M., Nethanel, T., Kaufmann, G., Sormunen, R., Läärä, E., Nasheuer, H. P., Rahmeh, A., Lee, M. Y.W.T., Syväoja, J., and Pospiech, H. (2007) 'Distinc­tive activities of DNA polymerases alpha, delta, and epsilon during human DNA replication'. FEBS JOURNAL, 273 :2984-3001. [Details]

Rytkonen, AK,Vaara, M,Nethanel, T,Kaufmann, G,Sormunen, R,Laara, E,Nasheuer, HP,Rahmeh, A,Lee, MYWT,Syvaoja, JE,Pospiech, H (2006) 'Distinctive activities of DNA polymerases during human DNA replication'. Febs Journal, 273 :2984-3001. [DOI] [Details]

Liu, PJ,Barkley, LR,Day, T,Bi, XH,Slater, DM,Alexandrow, MG,Nasheuer, HP,Vaziri, C (2006) 'The Chk1-mediated S-phase checkpoint targets initiation factor Cdc45 via a Cdc25A/Cdk2-independent mechanism'. Journal Of Biological Chemistry, 281 :30631-30644. [DOI] [Details]

Rytkonen, AK,Hillukkala, T,Vaara, M,Sokka, M,Jokela, M,Sormunen, R,Nasheuer, HP,Nethanel, T,Kaufmann, G,Pospiech, H,Syvaoja, JE (2006) 'DNA polymerase epsilon associates with the elongating form of RNA polymerase II and nascent transcripts'. Febs Journal, 273 :5535-5549. [DOI] [Details]

Weisshart, K,Pestryakov, P,Smith, RWP,Hartmann, H,Kremmer, E,Lavrik, O,Nasheuer, HP (2004) 'Coordinated regulation of replication protein A activities by its subunits p14 and p32'. Journal Of Biological Chemistry, 279 :35368-35376. [DOI] [Details]

Beyer, A,Hollunder, J,Nasheuer, HP,Wilhelm, T (2004) 'Post-transcriptional expression regulation in the yeast Saccharomyces cerevisiae on a genomic scale'. Molecular & Cellular Proteomics, 3 :1083-1092. [DOI] [Details]

Weisshart, K., Pestryakov, P., Smith, R. W., Hartmann, H., Kremmer, E., Lavrik, O., and Nasheuer, H.P. (2003) 'Coordinated regulation of replication protein A activities by its subunits p14 and p32'. J Biol Chem, 279 :35368-35376. [Details]

Weisshart, K., Friedl, S., Taneja, P., Nasheuer, H.P., Schlott, B., Grosse, F. and Fanning, E. (2003) 'Partial proteolysis of SV40 T antigen reveals intramolecular contacts between domains and conformation changes upon hexamer assembly'. J Biol Chem, 279 :38943-38951. [Details]

Pestryakov, PE,Weisshart, K,Schlott, B,Khodyreva, SN,Kremmer, E,Grosse, F,Lavrik, OI,Nasheuer, HP (2003) 'Human replication protein A - The C-terminal RPA70 and the central RPA32 domains are involved in the interactions with the 3 '-end of a primer-template DNA'. Journal Of Biological Chemistry, 278 :17515-17524. [DOI] [Details]

Wilhelm, T,Nasheuer, HP,Huang, S (2003) 'Physical and functional modularity of the protein network in yeast'. Molecular & Cellular Proteomics, 2 :292-298. [DOI] [Details]

Smith, RWP,Nasheuer, HP (2003) 'Initiation of JC virus DNA replication in vitro by human and mouse DNA polymerase alpha-primase'. European Journal Of Biochemistry, 270 :2030-2037. [DOI] [Details]

Pollok, S., Stoepel, J., Bauerschmidt, C., Kremmer, E., and Nasheuer, H.P. (2003) 'Regulation of eukaryotic DNA replication at the initiation step by Cdc45 and DNA polymerase α phosphorylation'. Biochemical Society Transactions, 31 :266-269. [Details]

Melle, C. and Nasheuer, H.P. (2002) 'Physical and functional interactions of the tumor suppressor protein p53 and DNA polymerase α-primase'. Nucleic Acids Res, 30 :1493-1499. [Details]

Smith, R.W.P., Steffen, C., Grosse, F., and Nasheuer, H.P. (2002) 'Species specificity of Simian Virus 40 DNA replication in vitro requires multiple functions of human DNA polymerase α'. J Biol Chem, 277 :20541-20548. [Details]

Smith, R.W.P. and Nasheuer, H.P. (2002) 'Control of complex formation of DNA polymerase α-primase and cell-free DNA replication by the C-terminal amino acids of the largest subunit p180?'. Febs Letters, 527 :143-146. [Details]

Schub, O., Rohaly, G., Smith, R.W.P., Schneider, A., Dehde, S., Dornreiter, I., and Nasheuer, H.P. (2001) 'Two independent phosphorylation sites of cellular DNA polymerase α-primase cooperate to regulate initiation of simian virus 40 DNA replication'. J Biol Chem, 276 :38076-38083. [Details]

Kautz, A. R., Weisshart, K., Schneider, A., Grosse, F., and Nasheuer, H.P. (2001) 'Aminoacids 257 to 288 of mouse p48 control the cooperation of polyomavirus large T antigen, replication protein A, and DNA polymerase α-primase to synthesize DNA in vitro'. J Virol, 75 :8569-8578. [Details]

Søe, K., Dianov, G., Nasheuer, H.P., Bohr, V. A., Grosse, F. and Stevnsner, T. (2001) 'A human topoisomerase I cleavage complex is recognized by an additional human topoisomerase I molecule in vitro'. Nucleic Acids Res, 29 :3195-3203. [Details]

Dehde, S., Rohaly, G., Schub, O., Nasheuer, H.P., Bohn, W. Chemnitz, J., Deppert, W., and Dornreiter, I. (2001) 'Two immunologically distinct human DNA polymerase α-primase subpopulations are involved in cellular DNA replication'. Mol Cell Biol, 21 :2581-2593. [Details]

Kautz, A. R., Schneider, A., Weisshart, K., Geiger, C., and Nasheuer, H.P. (2000) 'Different regions of primase subunit p48 control mouse polyomavirus and simian virus 40 DNA replication in vitro'. J Virol, 75 :1751-1760. [Details]

Kolpashchikov, D. M., Nasheuer, H.P., Weisshart, K., Ivanova, T. M., Bogachev, V. S., Favre, A., and Lavrik, O. I. (2000) 'Synthesis of base-substituted dUTP analogs carrying photoreactive 2-Nitro-5-azidobenzoyl group attached to 5'-position of uridine and its application to study human replication protein A by photoaffinity labeling'. Bioconjug. Chem, 11 :445-451. [Details]

Weisshart, K., Förster, H., Kremmer, E., Schlott, B., Grosse, F., and Nasheuer, H.P. (2000) 'Protein-protein interactions of the primase subunits p58 and p48 with simian virus 40 T antigen are required for efficient primer synthesis in a cell-free system'. J Biol Chem, 275 :17328-17337. [Details]

Kolpashchikov, D. M., Weisshart, K., Nasheuer, H.P., Khodyreva, S. N., Fanning, E., Favre, A., and Lavrik, O. I. (1999) 'Interaction of the RPA p70 subunit with a DNA template directs the p32 subunit to the 3´-end of nascent'. Febs Lett, 450 :131-134. [Details]

Voitenleitner, C., Rehfuess, C., Hilmes, M., O´Rear, L., Liao, P.-C., Gage, D. A., Ott, R., Nasheuer, H.P., and Fanning, E. (1999) 'Cell cycle-dependent regulation of human DNA polymerase α-primase activity by phosphorylation'. Mol Cell Biol, 19 :646-656. [Details]

Kühn, C., Müller, F., Melle, C., Nasheuer, H.P., Janus, F., Deppert, W., and Grosse F. (1999) 'Surface plasmon resonance measurements reveal stable complex formation between tumorsuppressor gene product p53 and DNA polymerase ?-primase'. Oncogene, 18 :769-774. [Details]

Lavrik O.I., Kolpashchikov D.M., Weisshart K., Nasheuer H.P., Khodyreva S. N., and Favre A. (1999) 'RPA subunit arrangement near the 3'-end of the primer is modulated by the length of the template strand and cooperative protein interactions'. Nucleic Acids Res, 27 :4235-4240. [Details]

Schneider, A., Smith, R. W. P., Kautz, A. R., Weisshart, K., Grosse, F. and Nasheuer, H. P. (1998) 'Primase activity of the DNA polymerase α-primase complex: Divalent cations stabilize the enzyme activity of the p48 subunit'. J Biol Chem, 273 :21608-21615. [Details]

Dantzer, F., Nasheuer, H. P., Vonesch, J.-L., de Murcia, G. and Ménissier-de Murcia, J. (1998) 'Poly ADP-ribose polymerase links the DNA damage surveillance network to the DNA replication machinery'. Nucleic Acids Res, 26 :1891-1898. [Details]

Lavrik, O.I., Kolpashchikov, D. M., Nasheuer, H. P., Weisshart, K. and Favre, A. (1998) 'Alternative RPA conformations are detected by crosslinks with primers carrying photoreactive group at 3'-end'. Febs Lett, 441 :186-190. [Details]

Lavrik, O. I., Nasheuer, H. P., Weisshart, K., Wold, M. S., Prasad, R., Beard, W. A., Wilson, A. H. and Favre, A. (1998) 'Subunits of human replication protein A are crosslinked by photoreactive primers synthesized by DNA polymerases'. Nucleic Acids Res, 26 :602-607. [Details]

Voitenleitner, C., Fanning, E., and Nasheuer, H. P. (1997) 'Phosphorylation of DNA polymerase α-primase by cyclin A-dependent kinases regulates initiation of DNA replication in vitro'. Oncogene, 14 :1611-1615. [Details]

Nesper, J., Smith, R. W. P., Kautz, A. R., Sock, E., Wegner, M., Grummt, F., and Nasheuer, H. P. (1997) 'A cell-free replication system for human polyomavirus'. J Virol, 71 :7421-7428. [Details]

Stadlbauer, F., Voitenleitner, C., Brückner, A., Fanning, E., and Nasheuer, H. P. (1996) 'Species-specific replication of simian virus 40 DNA in vitro requires the p180 subunit of human DNA polymerase α-primase'. Mol Cell Biol, 16 :94-104. [Details]

Brückner, A., Stadlbauer, F., Guarino, L. A., Brunahl, A., Schneider, C., Rehfuess, C., Prives, C., Fanning, E. and Nasheuer, H. P. (1995) 'The mouse DNA polymerase α-primase subunit p48 mediates species- specific replication of polyomavirus DNA in vitro'. Mol Cell Biol, 15 :1716-1724. [Details]

Stadlbauer, F., Brueckner, A., Rehfuess, C., Eckerskorn, C., Lottspeich, F., Förster, V., Tseng, B. Y. and Nasheuer, H. P. (1994) 'DNA replication in vitro by recombinant DNA-polymerase-α-primase'. Eur J Biochem, 222 :781-793. [Details]

Nasheuer, H. P., von Winkler, D., Schneider, C., Dornreiter, I., Gilbert, I., and Fanning, E. (1992) 'Purification and functional characterization of bovine RP-A in an in vitro SV40 DNA replication system'. Chromosoma, 102 :52-59. [Details]

Pearson, B. E., Nasheuer, H. P., and Wang, T. S. (1991) 'Human DNA polymerase α gene: sequences controlling expression in cycling and serum-stimulated cells'. Mol Cell Biol, 11 :2081-2095. [Details]

Nasheuer, H. P., Moore, A., Wahl, A. F. and Wang, T. S. (1991) 'Cell cycle-dependent phosphorylation of human DNA polymerase α'. J Biol Chem, 266 :7893-7903. [Details]

Bialek, G., Nasheuer, H. P., Goetz, H. and Grosse, F. (1989) 'Exonucleolytic proof-reading increases the accuracy of DNA synthesis by human lymphocyte DNA polymerase α-DNA primase'. Embo J, 8 :1833-1839. [Details]

Podust, V. N., Lavrik, O. I., Nasheuer, H. P. and Grosse, F. (1989) 'DNA polymerase α-DNA primase from human placenta. Immunoaffinity purification and preliminary characterization'. Febs Lett, 245 :14-16. [Details]

Bialek, G., Nasheuer, H. P., Goetz, H., Behnke, B. and Grosse, F. (1988) 'DNA polymerase α-DNA primase from human lymphoblasts'. Biochim Biophys Acta, 951 :290-297. [Details]

Nasheuer, H. P. and Grosse, F. (1988) 'DNA polymerase α-primase from calf thymus: Determination of the polypeptide responsible for primase activity'. J Biol Chem, 263 :8981-8988. [Details]

Nasheuer, H. P. and Grosse, F. (1987) 'Immunoaffinity-purified DNA polymerase α displays novel properties'. Biochemistry, 26 :8458-8466. [Details]

Grosse, F., Nasheuer, H. P., Scholtissek, S. and Schomburg, U. (1986) 'Lactate dehydrogenase and glyceraldehyde-phosphate dehydrogenase are single-stranded DNA-binding proteins that affect the DNA-polymerase-α-primase complex'. Eur J Biochem, 160 :459-467. [Details]

Freimüller-Kreutzer, B., Nasheuer, H. P. and Müller, W. H. (1985) 'Induction of alkaline phosphatase by retinoic acid'. Biol Chem Hoppe Seyler, 366 :317-321. [Details]

Other Journals

Broderick, R., and Nasheuer, H.P. (2009) 'Regulation of Cdc45 in the Cell Cycle and after DNA damage' Biochem Soc Trans 37 :926-930. [Details]

Oehlmann, M., Mahon, C., and Nasheuer, H.P. (2007) 'Comparison of DNA replication in Xenopus laevis and Simian Virus 40' Adv Exp Med Biol 604 :3-16. [Details]

Nasheuer, H.P., Smith, R. W. P., Bauerschmidt, C., Grosse, F., and Weisshart, K. (2002) 'Initiation of Eukaryotic DNA Replication - Regulation and Mechanisms' Prog Nucl Acid Res Mol Biol 72 :41-94. [Details]

Bauerschmidt, C., Kremmer, E., and Nasheuer, H.P. (2001) 'Initiation of Eukaryotic DNA-Replication - A Protein Network Is Decoded' Bioforum 24 :589-592. [Details]

Hübscher, U., Nasheuer, H.P., and Syväoja, J. (2000) 'Eukaryotic DNA polymerases, a growing family' Trends Biochem Sci 25 :143-147. [Details]

Hartmann, G. R., Biebricher, C., Glaser, S. J., Grosse, F., Katzameyer, M. J., Lindner, A. J., Mosig, H., Nasheuer, H.P., Rothman-Denes, L. B., Schäffner, A. R., Schnei¬der, G. J., Stetter, K.-O., and Thomm, M. (1988) 'Twelfth Fritz Lipmann lecture. Initiation of transcription--a general tool for affinity labeling of RNA polymerases by autocatalysis' Biol. Chem. Hoppe Seyler 369 :775-788. [Details]

Conference Publications

Broderick, R,Nasheuer, HP (2009) BIOCHEMICAL SOCIETY TRANSACTIONS Regulation of Cdc45 in the cell cycle and after DNA damage , pp.926-930 [DOI] [Details]

Oehlmann, M,Mahon, C,Nasheuer, HP (2007) ADVANCES IN MOLECULAR ONCOLOGY Comparison of DNA replication in Xenopus laevis and Simian Virus 40 , pp.3-16 [Details]

Pollok, S,Stoepel, J,Bauerschmidt, C,Kremmer, E,Nasheuer, HP (2003) BIOCHEMICAL SOCIETY TRANSACTIONS Regulation of eukaryotic DNA replication at the initiation step , pp.266-269 [Details]

 

Abstract

Fleming, RMT,Golden, A,Nasheuer, HP (2007) Thermodynamically constrained steady state solution space of the E-coli metabolic network. Abstract [DOI] [Details]

                                                                                                     

Reviews

Mahon, C,Liang, B,Tikhanovich, I,Abend, JR,Imperiale, MJ,Nasheuer, HP,Folk, WR (2009) Restriction of Human Polyomavirus BK Virus DNA Replication in Murine Cells and Extracts. Reviews [DOI] [Details]