Dr Jared Gerlach

Ph.D

Contact Details

Post Doctoral Researcher
T: Ext. 5885
E: jared.gerlach@nuigalway.ie
 
researcher

Biography

My research focuses on complex sugar structures (oligosaccharides and polysaccharides) and how they function in a variety of biological systems. Because they are part of every living organism, these complex sugars are vital to many aspects of health and often display variations under the influence of infection, disease or environmental stress.

My hands-on experience includes both fundamental and applied research in areas of glycomics, proteomics, biosensors and development of high-throughput screening and analysis techniques. As a member of the Glycoscience Group and the Regenerative Medicine Institute (REMEDI) at the National Centre for Biomedical Engineering Science (NCBES), I have been involved in academic collaborations as well as industrial partnerships with companies such as Randox Teoranta and Bristol-Myers Squibb. I participated in the Centre for Bioanalytical Sciences (CBAS), an international academic-industrial partnership, where I worked primarily on the rapid detection of minute quantities of enzymes in complex media. I also discovered and characterized fungal enzymes which facilitate the selective removal of oligosaccharides from proteins. Recently, I have studied the glycobiology of extracellular vesicles (also called exosomes, 20-100 nm vesicles released from cells) and their potential as diagnostic and prognostic indicators of renal health through an HRB-funded project with members of Mayo Clinic. I was also involved in the launch of Aquila Bioscience, Ltd., a spinout life-science company from NUI Galway.

Research Interests

As a biologist with experience spanning biochemistry and chemical biology, my research interests are diverse.

General areas of interest include:

Extracellular vesicles

Glycoconjugate characterization

Identification and control of pathogens

Novel carbohydrate-specific enzymes

Plant glycobiology

Enhancement of data sets for cross-discipline access and transfer

Automated and personalized diagnostics

Affinity-based analysis methods

Biosensors and applications of biosensing



 

Book Chapters

  Year Publication
(2009) 'Plant produced biopharmaceuticals'
Gerlach JQ, Kilcoyne M, McKeown P, Spillane C and Joshi L (2009) 'Plant produced biopharmaceuticals' In: Biotech Plants: Development, Utilization and Deployment. Berlin, Heidelberg, New York: Springer-Verlag. [Details]
(2009) 'GlycoSensors: Current Status and Potential'
Cunningham, S, Gerlach JQ and Joshi L (2009) 'GlycoSensors: Current Status and Potential' In: Biosensors: Properties, Materials and Applications. USA: NovaSciences. [Details]

Peer Reviewed Journals

  Year Publication
(2016) 'Temporal alterations in the bovine buttermilk glycome from parturition to milk maturation'
Ross, SA,Gerlach, JQ,Gill, SK,Lane, JA,Kilcoyne, M,Hickey, RM,Joshi, L (2016) 'Temporal alterations in the bovine buttermilk glycome from parturition to milk maturation'. Food Chemistry, 211 :329-338 [DOI] [Details]
(2016) 'Getting to know the extracellular vesicle glycome'
Gerlach, JQ,Griffin, MD (2016) 'Getting to know the extracellular vesicle glycome'. Molecular Biosystems, 12 :1071-1081 [DOI] [Details]
(2015) 'Extracellular vesicles from Paracoccidioides pathogenic species transport polysaccharide and expose ligands for DC-SIGN receptors'
da Silva, RP,Heiss, C,Black, I,Azadi, P,Gerlach, JQ,Travassos, LR,Joshi, L,Kilcoyne, M,Puccia, R (2015) 'Extracellular vesicles from Paracoccidioides pathogenic species transport polysaccharide and expose ligands for DC-SIGN receptors'. Scientific Reports, 5 [DOI] [Details]
(2015) 'APPLICATIONS OF NANOTECHNOLOGY IN ARRAY-BASED CARBOHYDRATE ANALYSIS AND PROFILING'
Gerlach, Jared Q and Kilcoyne, Michelle and Joshi, Lokesh (2015) 'APPLICATIONS OF NANOTECHNOLOGY IN ARRAY-BASED CARBOHYDRATE ANALYSIS AND PROFILING'. [Details]
(2015) 'Assessing Bacterial Interactions Using Carbohydrate-Based Microarrays'
Flannery, Andrea and Gerlach, Jared Q and Joshi, Lokesh and Kilcoyne, Michelle (2015) 'Assessing Bacterial Interactions Using Carbohydrate-Based Microarrays'. Microarrays, 4 (4):690-713 [Details]
(2014) 'Cross-platform comparison of glycan microarray formats'
Wang L, Cummings RD, Smith DF, Huflejt M, Campbell CT, Gildersleeve JC, Gerlach JQ, Kilcoyne M, Joshi L, Serna S, Reichardt NC, Parera Pera N, Pieters RJ, Eng W, Mahal LK (2014) 'Cross-platform comparison of glycan microarray formats'. Glycobiology, 24 (6):507-517 [DOI] [Details]
(2014) 'Campylobacter jejuni strain discrimination and temperature-dependent glycome expression profiling by lectin microarray'
Kilcoyne M, Twomey ME, Gerlach JQ, Kane M, Moran AP, Joshi L (2014) 'Campylobacter jejuni strain discrimination and temperature-dependent glycome expression profiling by lectin microarray'. Carbohydrate research, 389 :123-133 [DOI] [Details]
(2014) 'Microarray evaluation of the effects of lectin and glycoprotein orientation and data filtering on glycoform discrimination'
Gerlach, JQ,Kilcoyne, M,Joshi, L (2014) 'Microarray evaluation of the effects of lectin and glycoprotein orientation and data filtering on glycoform discrimination'. Analytical Methods, 6 :440-449 [DOI] [Details]
(2014) 'Profiling temporal changes in bovine milk lactoferrin glycosylation using lectin microarrays'
O'Riordan N, Gerlach JQ, Kilcoyne M, O'Callaghan J, Kane M, Hickey RM, Joshi L (2014) 'Profiling temporal changes in bovine milk lactoferrin glycosylation using lectin microarrays'. Food Chemistry, 165 :388-396 [DOI] [Details]
(2013) 'Surface Glycosylation Profiles of Urine Extracellular Vesicles'
Gerlach, JQ,Kruger, A,Gallogly, S,Hanley, SA,Hogan, MC,Ward, CJ,Joshi, L,Griffin, MD (2013) 'Surface Glycosylation Profiles of Urine Extracellular Vesicles'. Plos One, 8 [DOI] [Details]
(2013) 'Divergent Mechanisms of Interaction of Helicobacter pylori and Campylobacter jejuni with Mucus and Mucins'
Naughton, JA,Marino, K,Dolan, B,Reid, C,Gough, R,Gallagher, ME,Kilcoyne, M,Gerlach, JQ,Joshi, L,Rudd, P,Carrington, S,Bourke, B,Clyne, M (2013) 'Divergent Mechanisms of Interaction of Helicobacter pylori and Campylobacter jejuni with Mucus and Mucins'. Infection And Immunity, 81 :2838-2850 [DOI] [Details]
(2013) 'Donor Bone Marrow-derived Dendritic Cells Prolong Corneal Allograft Survival and Promote an Intragraft Immunoregulatory Milieu'
O'Flynn, L,Treacy, O,Ryan, AE,Morcos, M,Cregg, M,Gerlach, J,Joshi, L,Nosov, M,Ritter, T (2013) 'Donor Bone Marrow-derived Dendritic Cells Prolong Corneal Allograft Survival and Promote an Intragraft Immunoregulatory Milieu'. Molecular Therapy, 21 :2102-2112 [DOI] [Details]
(2012) 'Differential release of high mannose structural isoforms by fungal and bacterial endo-beta-N-acetylglucosaminidases'
Gerlach, JQ,Kilcoyne, M,Farrell, MP,Kane, M,Joshi, L (2012) 'Differential release of high mannose structural isoforms by fungal and bacterial endo-beta-N-acetylglucosaminidases'. Molecular Biosystems, 8 :1472-1481 [DOI] [Details]
(2012) 'Surface chemistry and linker effects on lectin-carbohydrate recognition for glycan microarrays'
Kilcoyne, M,Gerlach, JQ,Kane, M,Joshi, L (2012) 'Surface chemistry and linker effects on lectin-carbohydrate recognition for glycan microarrays'. Analytical Methods, 4 :2721-2728 [DOI] [ARAN Link] [Details]
(2012) 'Construction of a Natural Mucin Microarray and Interrogation for Biologically Relevant Glyco-Epitopes'
Kilcoyne, M,Gerlach, JQ,Gough, R,Gallagher, ME,Kane, M,Carrington, SD,Joshi, L (2012) 'Construction of a Natural Mucin Microarray and Interrogation for Biologically Relevant Glyco-Epitopes'. Analytical Chemistry, 84 :3330-3338 [DOI] [ARAN Link] [Details]
(2011) 'Periodic acid-Schiff's reagent assay for carbohydrates in a microtiter plate format'
Kilcoyne, M,Gerlach, JQ,Farrell, MP,Bhavanandan, VP,Joshi, L (2011) 'Periodic acid-Schiff's reagent assay for carbohydrates in a microtiter plate format'. Analytical Biochemistry, 416 :18-26 [DOI] [ARAN Link] [Details]
(2011) 'Non-carbohydrate-mediated interaction of lectins with plant proteins'
Gerlach JQ, Kilcoyne M, Eaton S, Bhavanandan V, Joshi L (2011) 'Non-carbohydrate-mediated interaction of lectins with plant proteins'. Advances In Tnf Family Research, 705 :257-269 [DOI] [Details]
(2010) 'Glycobiomimics and glycobiosensors'
Gerlach, JQ,Cunningham, S,Kane, M,Joshi, L (2010) 'Glycobiomimics and glycobiosensors'. Biochemical Society Transactions, 38 :1333-1336 [DOI] [Details]
(2010) 'Glyco-biosensors: recent advances and applications for the detection of free and bound carbohydrates'
Cunningham S, Gerlach JQ, Kane M, Joshi L (2010) 'Glyco-biosensors: recent advances and applications for the detection of free and bound carbohydrates'. Analyst, 135 (10):2471-2480 [DOI] [Details]
(2009) 'O-glycosylation of protein subpopulations in alcohol-extracted rice proteins'
Kilcoyne, M,Shah, M,Gerlach, JQ,Bhavanandan, V,Nagaraj, V,Smith, AD,Fujiyama, K,Sommer, U,Costello, CE,Olszewski, N,Joshi, L (2009) 'O-glycosylation of protein subpopulations in alcohol-extracted rice proteins'. Journal Of Plant Physiology, 166 :219-232 [DOI] [Details]
(2007) 'Label-free impedimetric detection of glycan-lectin interactions'
La Belle JT, Gerlach JQ, Svarovsky S, Joshi L (2007) 'Label-free impedimetric detection of glycan-lectin interactions'. Analytical Chemistry, 79 (18):6959-6964 [DOI] [Details]
(2006) 'Sensitive and rapid electrochemical bioassay of glycosidase activity'
Tangkuaram T, Gerlach JQ, Xiang Y, Kawde AN, Dai Z, Bhavanandan VP, La Belle JT, Veerasai W, Joshi L, Wang J (2006) 'Sensitive and rapid electrochemical bioassay of glycosidase activity'. Analyst, 131 (8):889-891 [DOI] [Details]
(2006) 'Nanoparticle-based sensing of glycan-lectin interactions'
Dai Z, Kawde AN, Xiang Y, La Belle JT, Gerlach J, Bhavanandan VP, Joshi L, Wang J (2006) 'Nanoparticle-based sensing of glycan-lectin interactions'. Journal Of The American Chemical Society, 128 (31):10018-10019 [DOI] [Details]

Conference Publications

  Year Publication
(2011) 2nd International symposium Microbes for health
Houeix B., Gerlach J.Q., Kilcoyne M., Kane M., Joshi L. (2011) Lectin profiling of commensal bacteria using lectin microarrays 2nd International symposium Microbes for health [Details]
(2010) AS
Gupta, S; Gerlach, J; Miura, Y; Yamazaki, T; Nakahara, T; Asada, H; Samali, A; Joshi, L. (2010) High Throughput Glycanmap (R) Analysis of Glycosylation during ER Stress and Apoptosis in a Colon Cancer Model AS [Details]
(2010) High Throughput Glycanmap (R) Analysis of Glycosylation during ER Stress and Apoptosis in a Colon Cancer Model
Sanjeev Gupta, Gerlach, J; Miura, Y; Yamazaki, T; Nakahara, T; Asada, H; Samali, A; Joshi, L. (2010) High Throughput Glycanmap (R) Analysis of Glycosylation during ER Stress and Apoptosis in a Colon Cancer Model [Details]

Abstract

  Year Publication
(2014) Construction and use of a mammalian lectin microarray for deciphering microbial-host and disease processes.
Kilcoyne, M,Utratna, M,Cunningham, S,Gerlach, J,Joshi, L (2014) Construction and use of a mammalian lectin microarray for deciphering microbial-host and disease processes. Abstract [Details]
(2014) High-throughput determination of non-human epitope Gal-alpha-(1 -> 3)-Gal on glycoproteins using a specific antibody fragment in a microarray format.
Kilcoyne, M,Cunningham, S,Gerlach, J,Joshi, L (2014) High-throughput determination of non-human epitope Gal-alpha-(1 -> 3)-Gal on glycoproteins using a specific antibody fragment in a microarray format. Abstract [Details]
(2011) Distinct Flow Cytometric Lectin-Binding Profiles of Urinary Exosomes and Purified Tamm-Horsfall Protein.
Gallogly, S,Kruger, A,Hanley, S,Gerlach, J,Hogan, M,Ward, C,Joshi, L,Griffin, M (2011) Distinct Flow Cytometric Lectin-Binding Profiles of Urinary Exosomes and Purified Tamm-Horsfall Protein. Abstract [Details]
(2011) Lectin Microarray Glycoprofiling of Intact Urine Exosomes and Tamm-Horsfall Protein.
Gerlach, J,Kruger, A,Hanley, S,Gallogly, S,Hogan, M,Ward, C,Griffin, M,Joshi, L (2011) Lectin Microarray Glycoprofiling of Intact Urine Exosomes and Tamm-Horsfall Protein. Abstract [Details]
(2008) Developing Novel Glycan Binding Agents.
Samli, KN,Gerlach, JQ,Kane, M,Joshi, L (2008) Developing Novel Glycan Binding Agents. Abstract [Details]

Conference Poster

  Year Publication
(2013) Profiling polysaccharides using lectin microarray technology and discrimination of structurally similar glycosaminoglycan.
Le Berre, M., Kilcoyne, M., Gerlach, J.Q., Kane, M., Joshi, L. (2013) Profiling polysaccharides using lectin microarray technology and discrimination of structurally similar glycosaminoglycan. Conference Poster [Details]

Meeting

  Year Publication
(2012) Glycosylation-Related Gene Expression Is Dysregulated in Multiple Myeloma and Overexpression of the Sialyltransferase ST3GAL6 Is Associated with Inferior Survival.
Glavey, SV,Cunningham, S,Murillo, LS,Loughrey, C,Wu, P,Cairns, M,Gill, SK,Kruger, A,Gerlach, JQ,Kane, M,Morgan, GJ,Joshi, L,O'Dwyer, ME (2012) Glycosylation-Related Gene Expression Is Dysregulated in Multiple Myeloma and Overexpression of the Sialyltransferase ST3GAL6 Is Associated with Inferior Survival. Meeting [Details]
(2010) A Comparative Glycan and Lectin Microarray Analysis of Virulent and Attenuated Strains of Campylobacter Jejuni at Host and Avian Temperatures.
Kilcoyne, M,Twomey, ME,Gerlach, JQ,Moran, AP,Joshi, L (2010) A Comparative Glycan and Lectin Microarray Analysis of Virulent and Attenuated Strains of Campylobacter Jejuni at Host and Avian Temperatures. Meeting [Details]

Presentation

  Year Publication
(2012) Lection Profiling of bacterial exopolysaccharides using microarray technology.
Le Berre M., Houeix B., Kilcoyne M., Gerlach J.Q., Kane M., Joshi L. (2012) Lection Profiling of bacterial exopolysaccharides using microarray technology. Presentation [Details]

Reviews

  Year Publication
(2010) Glyco-biosensors: Recent advances and applications for the detection of free and bound carbohydrates.
Cunningham, S,Gerlach, JQ,Kane, M,Joshi, L (2010) Glyco-biosensors: Recent advances and applications for the detection of free and bound carbohydrates. Reviews [DOI] [Details]