Research Interests

1. Production, characterization, molecular genetics and biotechnological application of eukaryotic glycoproteins and novel biocatalysts, with emphasis on glycosyl hydrolases, oxidoreductases and proteases. 2.Proteomics, structure-function analysis and cell biology of important cell-surface lectins/glycoproteins and enzymes. 3.Regulation of gene expression and glycoprotein secretion in filamentous fungi 4. Novel bioactive carbohydrates

 

 

Book Chapters

Tuohy, M.G., Buckley, R.J., Griffin, T.O., Connelly, I.C., Shanley, N.A., Filho, E.X.F., Hughes, M.M., Grogan, P. & Coughlan, M.P. (1989) 'Enzyme production by solid-state cultures of aerobic fungi on lignocellulosic substrates' In: M.P. Coughlan (eds). Enzyme systems for Lignocellulose Degradation. The Netherlands: Elsevier Applied Science. [Details]

Tuohy, M.G., Coughlan, T.L. & Coughlan, M.P. (1990) 'Solid-state versus liquid cultivation of Talaromyces emersonii on Straws and Pulps: Enzyme Productivity' In: M.P. Coughlan and M.T. Amaral-Collaço (eds). Advances in Biological Treatment of Lignocellulosic Materials. The Netherlands: Elsevier Applied Science. [Details]

Gillespie, A.-M., Keane, D., Griffin, T.O., Tuohy, M.G., Donaghy, J., Haylock, R.W. & Coughlan, M.P. (1990) 'The application of Fungal Enzymes in Flax Retting and the Properties of an Extracellular Polygalacturonase from Penicillium capsulatum' In: T. Kent Kirk and H.M. Chang (eds). Biotechnology in Pulp and Paper manufacture: Applications and Fundamental Investigations. USA: Butterworth-Heinemann. [Details]

Coughlan, M.P., Tuohy, M.G., Filho, E.X.F., Puls, J., Claeyssens, M., Vrsanska, M. & Hughes, M.M. (1993) 'Enzymological aspects of microbial hemicellulases with emphasis on fungal systems' In: Coughlan, M.P. and Hazlewood, G.P (eds). Hemicellulose and Hemicellulases. London and Chapel Hill: Portland Press. [Details]

Ryan, S.E., de Jong, E., Gubitz, G.M., Tuohy, M. & Saddler, J.N. (1998) 'The Potential of Hydrolytic Enzymes to modify Douglas-Fir derived Bleached Mechanical Pulps' In: Marc Claeyssens, Wim Nerinckx, Kathleen Piens (eds). Carbohydrases from Trichoderma reesei and other microorganisms: Structures, Biochemistry, Genetics and Applications. UK: The Royal Society of Chemistry. [Details]

Baillet, E., Downey, G. & Tuohy, M.G. (2003) 'Improvement of Texture and Volume in White Bread Rolls by incorporation of Microbial Hemicellulase Preparations' In: Courtin, C.M.; Veraverbeke, W.S.; Delcour, J.A (eds). Recent Advances in Enzymes in Grain Processing; Proceedings of the 3rd European Symposium on Enzymes in Grain Processing. Leuven, Belgium: Katholieke Universiteit Leuven. [Details]

Turner, K., Ayyachamy, M., Brebion, J., Gupta, V.K., and Tuohy, M.G. (2010) 'Fungal Enyzmes: A new route for the synthesis of Functional Prebiotics' In: Gupta, V.K.; Tuohy, M.; Gaur, R.K (eds). Fungal Biochemistry and Biotechnology. Germany: LAP Lambert Academic Publishing AG & Co. KG. [Details]

Manimaran Ayyachamy, Kevin Turner, Vijai Kumar Gupta and Maria G Tuohy. (2011) 'Lignin: An untapped resource for multiple industrial applications' In: Patersan, R.J (eds). Lignin: Properties and application in Biotechnology and Bioenergy. Nova Publishers, USA. [Details]

McCarthy, T. and Tuohy, M.G. (2011) 'A multi-step chromatographic strategy to purify three fungal endo-ß-glucanases. 2' In: Walls, D.; Loughran, S.T (eds). Methods Mol Biol.; Protein Chromatography: Methods and Protocols. New York USA: Springer. [DOI] [Details]

Gupta, V.K., Jain, P.K., Ayyachamy, M., Turner, K.M., O'Donovan, A. and Tuohy, M.G. (2011) 'Virulence of Fusarium spp. causing wilt disease of Guava with special reference to RNAi' In: Gaur, R.K.; Gafni, Y.; Sharma, P.; Gupta, V.K (eds). RNAi Technology. New Hampshire, USA: Science Publishers - CRC Press. [DOI] [Details]

V.K. Gupta, M. G. Tuohy and R. Gaur (2012) 'Methods for High Quality DNA Extraction from Fungi' In: V.K. Gupta and M.G. Tuohy (eds). Laboratory Protocols in Fungal Biology: Current Methods in Fungal Biology. New York, USA: Springer Science + Business Media LLC. [Details]

A. O’Donovan, V. K. Gupta, and M. G. Tuohy (2012) 'Fungal Specimen Collection and Processing' In: V.K. Gupta and M.G. Tuohy (eds). Laboratory Protocols in Fungal Biology: Current Methods in Fungal Biology. New York, USA: Springer Science + Business Media LLC. [Details]

M. Ayyachamy, V.K. Gupta, F.E. Cliffe, and M.G. Tuohy (2012) 'Enzymatic Saccharification of lignocellulosic biomass' In: V.K. Gupta and M.G. Tuohy (eds). Laboratory Protocols in Fungal Biology: Current Methods in Fungal Biology. New York, USA: Springer Science + Business Media LLC. [Details]

M. Ayyachamy, M. Shier, and M. G. Tuohy (2012) 'Large Scale Production of Lignocellulolytic Enzymes in Thermophilic Fungi' In: V.K. Gupta and M.G. Tuohy (eds). Laboratory Protocols in Fungal Biology: Current Methods in Fungal Biology. New York, USA: Springer Science + Business Media LLC. [Details]

V.K. Gupta, M. Tuohy, and A. Manimaran (2012) 'Role of Pathogenic Genes of Fusarium oxysporum, Coding Cell Wall Degrading Enzymes during Wilt Infection in Plants' In: Gupta, V.K. and Ayyachamy, M (eds). Biotechnology of Fungal Genes. USA: CRC Press. [Details]

Edited Books

V.K. Gupta, M. Tuohy, R.K. Gaur (Ed.). (2010) Fungal Biochemistry and Biotechnology. Germany: LAP Lambert Academic Publishing AG & Co. KG, Germany. [Details]

Gupta, V.K. and Tuohy, M.G (Ed.). (2012) Laboratory Protocols in Fungal Biology: Current Methods in Fungal Biology. New York, USA: Springer Science +Business Media, LLC. [Details]

Peer Reviewed Journals

Considine, P.J., Buckley, R.J., Griffin, T.O., Tuohy, M.G. & Coughlan, M.P. (1989) 'A simple and inexpensive method of solid-state cultivation'. Biotechnology Techniques, 3 :85-90. [DOI] [Details]

McCormack, J., Hackett, T.J., Tuohy, M.G. & Coughlan, M.P. (1991) 'Chitinase production by Talaromyces emersonii'. Biotechnology Letters, 13 :677-682. [Details]

Tuohy, M.G. & Coughlan. M.P. (1992) 'Production of Thermostable Xylan-Degrading Enzymes by Talaromyces emersonii'. Bioresource Technology, 39 :131-137. [Details]

Brooks,M.M.,Tuohy,M.G.,Savage, A.V.,Claeyssens, M.,Coughlan, M.P. (1992) 'The stereochemical course of reactions catalysed by the cellobiohydrolases produced by Talaromyces emersonii'. Biochemical Journal, 283 (1). [Details]

TUOHY, MG,PULS, J,CLAEYSSENS, M,VRSANSKA, M,COUGHLAN, MP (1993) 'THE XYLAN-DEGRADING ENZYME-SYSTEM OF TALAROMYCES-EMERSONII - NOVEL ENZYMES WITH ACTIVITY AGAINST ARYL BETA-D-XYLOSIDES AND UNSUBSTITUTED XYLANS'. Biochemical Journal, 290 :515-523. [Details]

TUOHY, MG,LAFFEY, CD,COUGHLAN, MP (1994) 'CHARACTERIZATION OF THE INDIVIDUAL COMPONENTS OF THE XYLANOLYTIC ENZYME-SYSTEM OF TALAROMYCES-EMERSONII'. Bioresource Technology, 50 :37-42. [Details]

Bartolome, B., Faulds., C.B., Tuohy, M.G. Hazlewood., G.P., Gilbert H.J. & G. Williamson (1995) 'Influence of different xylanases on the activity of ferulic acid esterase on wheat bran'. Biotechnology And Applied Biochemistry, 22 :65-73. [Details]

Mooney C.A., S.D. Mansfield, M.G. Tuohy, & J.N. Saddler (1998) 'The effect of initial pore volume and lignin content on the enzymatic hydrolysis of softwoods'. Bioresource Technology, 64 :113-119. [Details]

36. Thibault, J.-F., Asther, M., Colonna-Ceccaldi, B., Couteau, D., Delattre, M., Duarte, J.C., Faulds.C., Heldt-Hansen, H.-P., Kroon, P., Lesage-Meessen, L., Micard, V., Renard, C.M.G.C., Tuohy, M., Van Hulle, S. & Williamson, G. (1998) 'Fungal bioconversion of agricultural by-products to vanillin'. Lebensmittel-Wissenschaft Und-Technologie-Food Science And Technology, 31 :530-536. [Details]

Murray,P.G.,Grassick, A.,Laffey,C.D., Cuffe, M.M.,Higgins, T.,Savage, A.V.,Planas, A.,Tuohy, M.G. (2001) 'Isolation and characterization of a thermostable endo-beta-glucanase active on 1,3-1,4-beta-D-glucans from the aerobic fungus Talaromyces emersonii CBS 814.70'. Enzyme And Microbial Technology, 29 (1). [Details]

TUOHY, MG et al. (2002) 'Kinetic parameters and mode of action of the cellobiohydrolases produced by Talaromyces emersonii'. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULA, 1596 :366-380. [DOI] [Details]

Reen, F.J., Murray, P.G. & Tuohy, M.G. (2003) 'Molecular characterisation and expression analysis of the first hemicellulase gene (bxl1) encoding b -xylosidase from the thermophilic fungus Talaromyces emersonii'. Biochem Biophys Res Commun, 305 :579-585. [DOI] [Details]

Murray, P.G., Collins, C.M., Grassick, A. & Tuohy, M.G. (2003) 'Molecular cloning, transcriptional and expression analysis of the first cellulose gene (cbh2), encoding cellobiohydrolase II, from the moderately thermophilic fungus Talaromyces emersonii and structure prediction of the gene product'. Biochem Biophys Res Commun, 301 :280-286. [DOI] [Details]

Ryan, S.E., Nolan, K., Thompson, R., Gubitz, G.M., Savage, A.V. & Tuohy, M.G. (2003) 'Purification and characterization of a new low molecular weight endoxylanase from Penicillium capsulatum'. Enzyme Microbial Technol, 33 :775-785. [DOI] [Details]

Grassick, A., Birrane, G., Tuohy, M.G. Murray, P.G. & Higgins, T.M. (2003) 'Crystallisation and preliminary crystallographic analysis of the catalytic domain of cellobiohydrolase I from Talaromyces emersonii'. Acta Crystallog. Section D - Biological Crystallography, 59 :1283-1284. [DOI] [Details]

McCarthy, T., Hanniffy, O., Savage, A.V. & Tuohy, M.G. (2003) 'Catalytic properties and mode of action of three endo-beta-glucanases from Talaromyces emersonii on soluble beta-1,4- and beta-1,3;1,4-linked glucans'. Int. J. Biol. Macrmol, 33 :141-148. [Details]

Murray, P.G., Aro, N., Collins, C., Grassick, A., Penttilä, M., Saloheimo, M. & Tuohy, M. (2004) 'Expression in Trichoderma reesei and characterisation of a thermostable family 3 b -glucosidase from the moderately thermophilic fungus Talaromyces emersonii'. Prot Exp. Purif, 38 :248-257. [DOI] [Details]

Hennequart, F., O'Connell, E., Spence, J. & Tuohy, M.G. (2004) 'Brown Macro-algae'. Aqua Feeds: Formulation And Beyond, 1 (4):14-18. [Details]

Grassick, A.,Murray, P.G.,Thompson,R.,Collins, C.M.,Byrnes, L.,Birrane, G.,Higgins, T.M.,Tuohy, M.G. (2004) 'Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii'. European Journal of Biochemistry, 271 (22). [Details]

Maloney, A.P., Callan, S.M., Murray, P.G. & Tuohy, M.G. (2004) 'Mitochondrial malate dehydrogenase from the thermophilic, filamentous fungus Talaromyces emersonii. Purification of the native enzyme, cloning and overexpression of the corresponding gene'. Eur J Biochem, 271 :3115-3126. [Details]

McCarthy, T., Hanniffy, O., Lalor, E., Savage, A.V. & Tuohy, M.G. (2005) 'Evaluation of three thermostable fungal endo-beta-glucanases from Talaromyces emersonii for brewing and food applications'. Proc. Biochem, 40 :1741-1748. [DOI] [Details]

McCarthy TC, Lalor E, Hanniffy O, Savage AV, Tuohy MG. (2005) 'Comparison of wild-type and UV-mutant beta-glucanase-producing strains of Talaromyces emersonii with potential in brewing applications'. J Ind Microbiol Biotechnol, 32 (4):125-134. [DOI] [Details]

M.N. Heneghan, L. McLoughlin, P.G. Murray & M.G. Tuohy (2007) 'Cloning, characterisation and expression analysis of a-glucuronidase from the thermophilic fungus Talaromyces emersonii'. Enzyme Microbial. Technol, 41 :677-682. [Details]

C.M. Collins, P.G. Murray, S. Denman, J.P. Morrissey, L. Byrnes, T.T. Teeri & M.G. Tuohy (2007) 'Molecular cloning and expression analysis of two distinct ß-glucosidase genes, bg1 and aven1, with very different biological roles from the thermophilic, saprophytic fungus Talaromyces emersonii'. Mycol. Res, 111 :840-849. [Details]

S. Fernandes, P.G Murray and M. G. Tuohy (2008) 'Enzyme systems from the thermophilic fungus Talaromyces emersonii for sugar beet bioconversion'. Bioresources, 3 :898-909. [Details]

O'Donoghue AJ, Mahon CS, Goetz DH, O'Malley JM, Gallagher DM, Zhou M, Murray PG, Craik CS, Tuohy MG. (2008) 'Inhibition of a secreted glutamic peptidase prevents growth of the fungus Talaromyces emersonii'. J Biol Chem, 283 :29186-29195. [DOI] [Details]

E. O'Connell, P. Murray, C. Piggott, F. Hennequart & M. Tuohy (2008) 'Purification and characterization of a N-acetylglucosaminidase produced by Talaromyces emersonii during growth on algal fucoidan'. J. Appl. Phycol, 20 :557-565. [DOI] [Details]

Forbes C, O'Reilly C, McLaughlin L, Gilleran G, Tuohy M, Colleran E. (2009) 'Application of high rate, high temperature anaerobic digestion to fungal thermozyme hydrolysates from carbohydrate wastes'. Water Res, 43 (9):2531-2539. [DOI] [Details]

Mahon CS, O'Donoghue AJ, Goetz DH, Murray PG, Craik CS, Tuohy MG (2009) 'Characterization of a multimeric, eukaryotic prolyl aminopeptidase: an inducible and highly specific intracellular peptidase from the non-pathogenic fungus Talaromyces emersonii'. Microbiology, 155 :3673-3682. [DOI] [Details]

Fernandes, S., Tuohy, M.G., Murray, P.G. (2009) 'Xylose reductase from the thermophilic fungus Talaromyces emersonii: cloning and heterologous expression of the native gene (Texr) and a double mutant (TexrK271R + N273D) with altered coenzyme specificity'. J. Biosci, 34 :881-890. [DOI] [Details]

Popper ZA and Tuohy MG (2010) 'Beyond the green: understanding the evolutionary puzzle of plant and algal cell walls'. Plant Physiol, 153 :373-383. [DOI] [Details]

Simila J, Gernig A, Murray P, Fernandes S and Tuohy MG (2010) 'Cloning and expression of a thermostable alpha-galactosidase from the thermophilic fungus Talaromyces emersonii in the methylotrophic yeast Pichia pastoris'. J. Microbiol. Biotechnol, 20 :1653-1663. [DOI] [Details]

Voutilainen S.P., Murray P.G., Tuohy M.G., Koivula A. (2010) 'Expression of Talaromyces emersonii cellobiohydrolase Cel7A in Saccharomyces cerevisiae and rational mutagenesis to improve its thermostability and activity'. Protein Eng Des Sel, 23 :69-79. [DOI] [Details]

Waters DM, Murray PG, Ryan LA, Arendt EK, Tuohy MG (2010) 'Talaromyces emersonii thermostable enzyme systems and their applications in wheat baking systems'. J. Agric. Food Chem, 58 :7415-7422. [DOI] [Details]

Fernandes, S., Tuohy, M.G., Murray, P.G. (2010) 'Cloning, Heterologous Expression, and Characterization of the Xylitol and L-Arabitol Dehydrogenase Genes, Texdh and Telad,from the Thermophilic Fungus Talaromyces emersonii'. Biochem. Genet, 48 :480-495. [DOI] [Details]

Rawson, A., Koidis, A., Brunton, N., Patras A., Tuohy, M. (2010) 'Modelling the effect of water immersion thermal processing on polyacetylene levels and instrumental colour of carrot disks'. Food Chemistry, 121 :62-68. [DOI] [Details]

Rawson, A., Koidis, A., Rai, D.K., Tuohy, M., Brunton, N (2010) 'Influence of Sous Vide and water immersion processing on polyacetylene content and instrumental color of parsnip (Pastinaca sativa) disks'. Journal of Agricultural and Food Chemistry, 58 :7740-7747. [DOI] [Details]

Gilleran, CT, Hernon AT, Murray PG and Tuohy MG (2010) 'Induction of Enzyme cocktails by low cost carbon sources for production of monosaccharide-rich syrups from plant materials'. Bioresources, 5 :634-649. [Details]

Waters DM, Ryan LA, Murray PG, Arendt EK, Tuohy MG. (2011) 'Characterization of a Talaromyces emersonii Thermostable Enzyme Cocktail with Applications in Wheat Dough Rheology'. Enzyme Microb. Technol, 49 :229-236. [DOI] [Details]

Foley SA, Mulloy B, Tuohy MG (2011) 'An unfractionated fucoidan from Ascophyllum nodosum: extraction, characterization, and apoptotic effects in vitro'. Journal Of Natural Products, 74 (9):1851-1861. [DOI] [Details]

Zöe A. Popper, Gurvan Michel, Cécile Hervé, David S. Domozych, William G.T.Willats, Maria G. Tuohy, Bernard Kloareg, and Dagmar B. Stengel (2011) 'Evolution and Diversity of Plant Cell Walls: From Algae to Flowering Plants'. Annu. Rev. Plant Biol, 62 :8.1-8.24. [Details]

O'Connell E, Piggott C and Tuohy M (2011) 'Purification of exo-1,3-beta-glucanase, a new extracellular glucanolytic enzyme from Talaromyces emersonii'. Appl. Microbiol. Biotechnol, 89 :685-696. [DOI] [Details]

Rawson, A., Hossain, M. B., Patras, A., Tuohy, M., Brunton, N (2011) 'Effect of boiling and roasting on the polyacetylene and polyphenol content of Fennel (Foeniculum vulgare) bulb'. Food Research International, . [Details]

Rawson, A., Tiwari, B.K., Tuohy, M., O'Donnell, C.P., Brunton, N (2011) 'Effect of ultrasound and blanching pretreatments on polyacetylene and carotenoid content of hot air and freeze dried carrot discs'. Ultrasonic Sonochemistry, 18 :1172-1179. [DOI] [Details]

Ashish Rawsona, Nigel Brunton, Maria Tuohy (2012) 'High pressure-temperature degradation kinetics of polyacetylenes in carrots'. Food Chemistry, 133 (1):15-20. [DOI] [Details]

Anastasios Koidis, Ashish Rawson, Maria Tuohy, Nigel Brunton (2012) 'Influence of unit operations on the levels of polyacetylenes in minimally processed carrots and parsnips: An industrial trial'. Food Chemistry, 132 (3):1406-1412. [DOI] [Details]

Deborah Waters, Patrick Murray, Yuta Miki, Angel Martinez, Maria Tuohy, and Craig Faulds (2012) 'A thermostable fungal acetylxylan esterase from Talaromyces emersonii: Cloning, over-expression in Escherichia coli and characterization'. Applied And Environmental Microbiology, . [Details]

Ashish Rawson, Brijesh K. Tiwari, Maria Tuohy, Nigel Brunton (2012) 'Impact of frozen storage on polyacetylene content, texture and colour in carrots disks'. Journal Of Food Engineering, 108 (4):563-569. [DOI] [Details]

 

Conference Publications

TUOHY, MG et al. (1989) ENZYME-PRODUCTION BY SOLID-STATE CULTURES OF AEROBIC FUNGI ON LIGNOCELLULOSIC SUBSTRATES ENZYME SYSTEMS FOR LIGNOCELLULOSE DEGRADATION , pp.293-312 [Details]

Gillespie, A.-M., Keane, D., Griffin, T.O., Tuohy, M.G., Donaghy, J., Haylock, R.W. & Coughlan, M.P. (1989) Properties of an extracellular polygalacturonase from Pencillium capsulatum and applications of the pectin-degrading system in flax retting 4th Int. Conf. on Biotechnol. in the Pulp and Paper Industry , pp.63-64 [Details]

M.G. Tuohy, and Coughlan, M.P. (1991) Production of thermostable xylan-degrading enzymes by Talaromyces emersonii Int. Energy Agency Symp.,Biotechnology for the Conversion of Lignocellulosics [Details]

Filho, E.X.F., Tuohy, M.G., Puls, J., & Coughlan, M.P. (1991) The xylan-degrading enzyme systems of Penicillium capsulatum and Talaromyces emersonii Biochemical Society , pp.Vol. 19, 25S-Vol. 19, 25S [Details]

Ryan, S.E., de Jong, E., Gubitz, G., Tuohy, M. & Saddler, J.N (1997) The potential of hydrolytic enzymes to modify Douglas-fir derived bleached mechanical pulp TRICEL Ghent, Belgium, , pp.214-216 [Details]

Mooney, C.A., S.D. Mansfield, M.G. Tuohy, &J.N. Saddler (1997) The effect of lignin content on cellulose accessibility and enzymatic hydrolysis of softwood pulps TAPPI Biological Sciences Symposium San Francisco, CA, , pp.259-265 [Details]

Ryan, S.E., de Jong, E., Gubitz, G., Tuohy, M. & Saddler, J.N. (1998) Enzymic modification of pretreated Douglas fir refiner pulp 7th Int. Conf. Biotechnol. Pulp Pap. Ind , pp.C147-C150 [Details]

Hayes, C., Collins, C., Murray, P.G., Tuohy, M.G., Griffin, T.O. & Higgins, T. (2000) Bioinformatics as a tools in generating sequence databases and investigating Enzyme Scaffolding Euchem. Conf., Stockholm [Details]

Gilleran, C.T., Murray, P.G. & Tuohy, M.G. (2002) Paper waste saccharification by the thermophilic aerobic fungus Talaromyces emersonii CBS814.70 ECRP 2002, The 8th European Roundtable on Cleaner Production Cork, Ireland, [Details]

 

Abstract

TUOHY, MG,PULS, J,CLAEYSSEN, M,VRSANSKA, M,COUGHLAN, MP (1994) PRODUCTION OF EXO-GLYCOSIDASE ENZYMES BY TALAROMYCES-EMERSONII CBS-81470 AND THEIR IMPORTANCE IN THE OVERALL HYDROLYSIS OF CELL-WALL POLYSACCHARIDES. Abstract [Details]

Tuohy, MG,Murray, PG,Puls, J,Casey, AL,Hennink, H (1999) Xylanases and mannanases from thermophilic fungi. Abstract [Details]