Shirley Hanley

Research Funded Snr Technician BSc, MSc, PhD.

Contact Details

Senior Technical Officer In Flow Cytometry
NCBES - CORES
Flow Cytometry Facility,
2nd Floor South
Biosciences Building
NUI Galway

T: 091 495825
E: shirley.hanley@nuigalway.ie
 
researcher

Biography

My research background is in molecular microbiology of both human and plant pathogens. I have also worked in Government testing laboratories as well as manufacturing industries specifically the medical device industry. 
I joined NUI Galway in 2008 as a Senior Technical Officer for the Immunology and Transplant Biology Group and since June 2011 I have been employed as a Senior Technical Officer in the NCBES with responsibility of managing the Flow Cytometry Core Facility.
My specific area of technical expertise is flow cytometry and flourescence activated cell sorting.

Research Interests

Peer Reviewed Journals

  Year Publication
(2014) 'The influence of hypoxia on the differentiation capacities and immunosuppressive properties of clonal mouse mesenchymal stromal cell lines'
Prado-Lopez, S,Duffy, MM,Baustian, C,Alagesan, S,Hanley, SA,Stocca, A,Griffin, MD,Ceredig, R (2014) 'The influence of hypoxia on the differentiation capacities and immunosuppressive properties of clonal mouse mesenchymal stromal cell lines'. Immunol Cell Biol, 92 :612-623 [DOI] [Details]
(2014) 'Mesenchymal stem cells and a vitamin D receptor agonist additively suppress T helper 17 cells and the related inflammatory response in the kidney'
Duffy, MM,McNicholas, BA,Monaghan, DA,Hanley, SA,McMahon, JM,Pindjakova, J,Alagesan, S,Fearnhead, HO,Griffin, MD (2014) 'Mesenchymal stem cells and a vitamin D receptor agonist additively suppress T helper 17 cells and the related inflammatory response in the kidney'. American Journal Of Physiology-Renal Physiology, 307 :1412-1426 [DOI] [Details]
(2013) 'Surface Glycosylation Profiles of Urine Extracellular Vesicles'
Gerlach, JQ,Kruger, A,Gallogly, S,Hanley, SA,Hogan, MC,Ward, CJ,Joshi, L,Griffin, MD (2013) 'Surface Glycosylation Profiles of Urine Extracellular Vesicles'. Plos One, 8 [DOI] [Details]
(2012) 'Interleukin-1 accounts for intrarenal Th17 cell activation during ureteral obstruction'
Pindjakova, J,Hanley, SA,Duffy, MM,Sutton, CE,Weidhofer, GA,Miller, MN,Nath, KA,Mills, KHG,Ceredig, R,Griffin, MD (2012) 'Interleukin-1 accounts for intrarenal Th17 cell activation during ureteral obstruction'. Kidney International, 81 :379-390 [DOI] [Details]
(2011) 'Developing Cell-Specific Antibodies to Endothelial Progenitor Cells Using Avian Immune Phage Display Technology'
Bowes, T,Hanley, SA,Liew, A,Eglon, M,Mashayekhi, K,O'Kennedy, R,Barry, F,Taylor, WR,O'Brien, T,Griffin, MD,Finlay, WJJ,Greiser, U (2011) 'Developing Cell-Specific Antibodies to Endothelial Progenitor Cells Using Avian Immune Phage Display Technology'. Journal of biomolecular screening, 16 :744-754 [DOI] [Details]
(2011) 'Mesenchymal stem cell inhibition of T-helper 17 cell- differentiation is triggered by cell-cell contact and mediated by prostaglandin E2 via the EP4 receptor'
Duffy MM, Pindjakova J, Hanley SA, McCarthy C, Weidhofer GA, Sweeney EM, English K, Shaw G, Murphy JM, Barry FP, Mahon BP, Belton O, Ceredig R, Griffin MD (2011) 'Mesenchymal stem cell inhibition of T-helper 17 cell- differentiation is triggered by cell-cell contact and mediated by prostaglandin E2 via the EP4 receptor'. European Journal Of Immunology, 41 (10):2840-2851 [DOI] [Details]
(2007) 'The rag locus of Porphyromonas gingivalis contributes to virulence in a murine model of soft tissue destruction'
Shi XJ (Shi, Xiaoju), Hanley SA (Hanley, Shirley A.), Faray-Kele MC (Faray-Kele, Marie-Claire), Fawell SC (Fawell, Stuart C.), Aduse-Opoku J (Aduse-Opoku, Joseph), Whiley RA (Whiley, Robert A.), Curtis MA (Curtis, Michael A.), Hall LMC (Hall, Lucinda M. C.) (2007) 'The rag locus of Porphyromonas gingivalis contributes to virulence in a murine model of soft tissue destruction'. JOURNAL OF IMMUNOLOGY, 75 (4):2071-2074 [Details]
(2001) 'Evaluation of a PCR/DNA probe colorimetric membrane assay for identification of Campylobacter spp. in human stool specimens'
Collins, E,Glennon, M,Hanley, S,Murray, AM,Cormican, M,Smith, T,Maher, M (2001) 'Evaluation of a PCR/DNA probe colorimetric membrane assay for identification of Campylobacter spp. in human stool specimens'. Journal Of Clinical Microbiology, 39 :4163-4165 [Details]
(2000) 'Identification of ragAB as a temperature-regulated operon of Porphyromonas gingivalis W50 using differential display of randomly primed RNA'
Bonass WA, Marsh PD, Percival RS, Aduse-Opoku J, Hanley SA, Devine DA, Curtis MA (2000) 'Identification of ragAB as a temperature-regulated operon of Porphyromonas gingivalis W50 using differential display of randomly primed RNA'. Infection And Immunity, 68 (7):4012-4017 [Details]
(1999) 'The rag locus of Porphyromonas gingivalis: a novel pathogenicity island'
Curtis MA, Hanley SA, Aduse-Opoku J (1999) 'The rag locus of Porphyromonas gingivalis: a novel pathogenicity island'. Journal of periodontal research, 34 (7):400-405 [Details]
(1999) 'A 55-kilodalton immunodominant antigen of Porphyromonas gingivalis W50 has arisen via horizontal gene transfer'
Hanley SA, Aduse-Opoku J, Curtis MA (1999) 'A 55-kilodalton immunodominant antigen of Porphyromonas gingivalis W50 has arisen via horizontal gene transfer'. Infection And Immunity, 67 (3):1157-1171 [Details]

Conference Contributions

  Year Publication
(2011) Glycobiology 2011,
J. Gerlach, A. Krüger, S. Hanley, S. Gallogly, M. Hogan, C. Ward, M. Griffin, L. Joshi (2011) Lectin Microarray Glycoprofiling of Intact Urine Exosomes and Tamm-Horsfall Protein. [Poster Presentation], Glycobiology 2011, USA , 01-NOV-11 - 01-NOV-11. [Details]
(2011) Annual Meeting American Society for Nephrology,
J. Gerlach, A. Krüger, S. Hanley, S. Gallogly, M. Hogan, C. Ward, L. Joshi, M. Griffin (2011) Lectin Microarrays of Intact Urinary Exosomes Indicate Distinct Glycosylation Profiles. [Poster Presentation], Annual Meeting American Society for Nephrology, Philadelphia, USA , 01-NOV-11 - 01-NOV-11. [Details]
(2011) Glycobiology 2011,
S. Gallogly, A. Krüger, S. Hanley, J. Gerlach, M. Hogan, C. Ward, L. Joshi, M. Griffin (2011) Distinct Flow Cytometric Lectin-Binding Profiles of Urinary Exosomes and Purified Tamm-Horsfall Protein. [Poster Presentation], Glycobiology 2011, USA , 01-NOV-11 - 01-NOV-11. [Details]

Abstract

  Year Publication
(2011) Lectin Microarray Glycoprofiling of Intact Urine Exosomes and Tamm-Horsfall Protein.
Gerlach, J,Kruger, A,Hanley, S,Gallogly, S,Hogan, M,Ward, C,Griffin, M,Joshi, L (2011) Lectin Microarray Glycoprofiling of Intact Urine Exosomes and Tamm-Horsfall Protein. Abstract [Details]
(2011) Distinct Flow Cytometric Lectin-Binding Profiles of Urinary Exosomes and Purified Tamm-Horsfall Protein.
Gallogly, S,Kruger, A,Hanley, S,Gerlach, J,Hogan, M,Ward, C,Joshi, L,Griffin, M (2011) Distinct Flow Cytometric Lectin-Binding Profiles of Urinary Exosomes and Purified Tamm-Horsfall Protein. Abstract [Details]

Meeting

  Year Publication
(2012) Circulating Human Monocytes differ in Phenotype and Function According to Metabolic Profile in Obesity and Type 2 Diabetes Mellitus.
Dennedy, MC,Lopez, SP,Hanley, S,Connaughton, E,Slevin, S,Ceredig, R,O'Shea, D,O'Brien, T,Griffin, M (2012) Circulating Human Monocytes differ in Phenotype and Function According to Metabolic Profile in Obesity and Type 2 Diabetes Mellitus. Meeting [Details]
(2010) Mesenchymal Stromal Cells (MSCs) Potently Inhibit the Primary Induction of T-Helper 17 Cells through Cell-Cell Contact.
Duffy, M,Pindjakova, J,Weidhofer, G,Hanley, S,Mahon, BP,Ceredig, R,Griffin, M (2010) Mesenchymal Stromal Cells (MSCs) Potently Inhibit the Primary Induction of T-Helper 17 Cells through Cell-Cell Contact. Meeting [Details]

Professional Associations

  Association Function From / To
Irish Cytometry Society member of the annual ICyS conference organising committee /
The International Society for the Advancement of Cytometry member /

Employment

  Employer Position From / To
NUIG Senior Technical Officer in Flow Cytometry 01-JUN-12 /
NUIG Senior Technical Officer 14-JAN-08 / 31-DEC-99
Medtronic QA Sterilsation Engineer 01-FEB-07 / 30-SEP-07
Beckman Coulter Technical Operations Scientist 04-JAN-05 / 30-JAN-07
The State Laboratory Molecular Biologist 04-JAN-01 / 31-DEC-05
The National Diagnostic Centre Postdoctoral Scientist 04-JAN-99 / 31-DEC-00

Education

  Year Institution Qualification Subject
2004 Irish Management Institute Certificate Supervisory Management
1998 University of London
1993 University of Galway
1994 University of Leicester

Teaching Interests

Course lecturer and co-ordinator of the flow cytometry module and practical demonstration for the MSc Regenerative Medicine and MSc Biomedical Science taught Masters programmes; course lecturer for the MSc Cancer Research.