Dr Enda O'Connell BSc, Ph.D
Enda joined the National Centre for Biomedical Engineering Science in 2005 from the School of Biomolecular and Biomedical Science and Conway Institute of Biomolecular and Biomedical Research at University College Dublin. He holds a B.Sc. in Industrial Microbiology and a Ph.D. in Molecular Microbiology from UCD, where he studied the regulation of virulence gene expression in the intracellular equine pathogen Rhodococcus equi.
In his role as Senior Technical Officer, Enda provides training and support for researchers in Functional Genomics and Molecular Biology techniques, to examine gene expression and regulation. These include Microarray and Real Time PCR technology, RNA Isolation and QC, Experimental Design and Microarray Data Analysis using the GeneSpring GX data analysis package.
Enda also provides support for the recently established NCBES High Throughput Screening facility, where Drug Discovery and Characterisation, Cellular, Enzymatic and Binding Assays, as well as Sample Analysis may be performed. Enda's responsibilities include, Assay Design, Development and Validation, Operation of the Robotic Equipment and Primary Screening.
Gene Expression Profiling by Microarray and qPCR analysis.
|Prasad BR, O'Connell E, Smith TJ, GÃ©rard VA, Gun'ko YK, Rochev Y (2012) 'Evaluating the potential of quantum dots for in vitro biological studies: effects on gene expression using microarray analysis' In: Nanoparticles in Biology and Medicine: Methods and Protocols. Clifton, N.J: Methods in Molecular Biology. [DOI] [Details]|
Peer Reviewed Journals
|FitzGerald J, Murillo LS, O'Brien G, O'Connell E, O'Connor A, Wu K, Wang GN, Rainey MD, Natoni A, Healy S, O'Dwyer M, Santocanale C (2014) 'A high through-put screen for small molecules modulating MCM2 phosphorylation identifies Ryuvidine as an inducer of the DNA damage response'. Plos One, 9 (6). [DOI] [Details]|
|Monaghan D, O'Connell E, Cruickshank FL, O'Sullivan B, Giles FJ, Hulme AN, Fearnhead HO (2014) 'Inhibition of protein synthesis and JNK activation are not required for cell death induced by anisomycin and anisomycin analogues'. Biochem, 443 (2):761-767. [DOI] [Details]|
|Islam, N,Whitehouse, M,Mehendale, S,Hall, M,Tierney, J,O'Connell, E,Blom, A,Bannister, G,Hinde, J,Ceredig, R,Bradley, BA (2014) 'Post-traumatic immunosuppression is reversed by anti-coagulated salvaged blood transfusion: deductions from studying immune status after knee arthroplasty'. Clinical And Experimental Immunology, 177 :509-520. [DOI] [Details]|
|Toner, AP,McLaughlin, F,Giles, FJ,Sullivan, FJ,O'Connell, E,Carleton, LA,Breen, L,Dunne, G,Gorman, AM,Lewis, JD,Glynn, SA (2013) 'The novel toluidine sulphonamide EL102 shows pre-clinical in vitro and in vivo activity against prostate cancer and circumvents MDR1 resistance'. British Journal Of Cancer, 109 :2131-2141. [DOI] [Details]|
|Flynn AW, Chen X, O'Connell E, O'Brien T (2012) 'A comparison of the efficacy of transplantation of bone marrow derived mesenchymal stem cells and unrestricted somatic stem cells on outcome after acute myocardial infarction'. Stem Cell Research & Therapy, 3 (5). [DOI] [Details]|
|Stocca, A,O'Toole, D,Hynes, N,Hynes, SO,Mashayekhi, K,McGinley, L,O'Connell, E,Coleman, C,Sultan, S,Duffy, A,Tunev, S,O'Brien, T (2012) 'A Role for MRP8 in in stent restenosis in diabetes'. Atherosclerosis, 221 :325-332. [DOI] [Details]|
|FitzGerald J, Moureau S, Drogaris P, O'Connell E, Abshiru N, Verreault A, Thibault P, Grenon M, Lowndes NF. (2011) 'Regulation of the DNA damage response and gene expression by the Dot1L histone methyltransferase and the 53Bp1 tumour suppressor'. Plos One, 6 (2). [DOI] [Details]|
|Miranda-Casoluengo R, Miranda-Casoluengo AA, O'Connell EP, Fahey RJ, Boland CA, VÃ¡zquez-Boland JA, Meijer WG (2011) 'The vapA co-expressed virulence plasmid gene vcgB (orf10) of the intracellular actinomycete Rhodococcus equi'. Microbiology, 157 (Pt 8):2357-2368. [DOI] [Details]|
|Cunnea, P,McMahon, J,O'Connell, E,Mashayekhi, K,Fitzgerald, U,McQuaid, S (2010) 'Gene expression analysis of the microvascular compartment in multiple sclerosis using laser microdissected blood vessels'. Acta Neuropathologica, 119 :601-615. [DOI] [Details]|
|Byrne GA, Boland CA, O'Connell EP, Meijer WG (2008) 'Differential mRNA stability of the vapAICD operon of the facultative intracellular pathogen Rhodococcus equi'. Fems Microbiology Letters, 280 (1):89-94. [DOI] [Details]|
|Gundy S, Manning G, O'Connell E, EllÃ¤ V, Harwoko MS, Rochev Y, Smith T, Barron V (2008) 'Human coronary artery smooth muscle cell response to a novel PLA textile/fibrin gel composite scaffold'. Acta Biomaterialia, 4 (6):1734-1744. [DOI] [Details]|
|Miranda-Casoluengo R, Duffy PS, O'Connell EP, Graham BJ, Mangan MW, Prescott JF, Meijer WG (2005) 'The iron-regulated iupABC operon is required for saprophytic growth of the intracellular pathogen Rhodococcus equi at low iron concentrations'. Journal Of Bacteriology, 187 (10):3438-3444. [DOI] [Details]|
|Russell DA, Byrne GA, O'Connell EP, Boland CA, Meijer WG (2004) 'The LysR-type transcriptional regulator VirR is required for expression of the virulence gene vapA of Rhodococcus equi ATCC 33701'. Journal Of Bacteriology, 186 (17):5576-5584. [DOI] [Details]|
|Fitzgerald, J; Murillo, L; O’Brien, G; O’Connell, E; O’Connor, A; Wu, K; Wang, GN; Rainey, MD; Natoni, A; Healy, S; O’Dwyer, M; Santocanale, C (2014) A high through put screen identifies Ryuvidine as a modulator of Cdc7 dependent phosphorylation of MCM2 and an inducer of the DNA damage response Irish Association for Cancer Research [Details]|
|Ali, H; Burke, A; O’Connell, E; Giles, FJ; Sullivan, F; Glynn, S. (2014) Novel Kinase Inhibitors as Drug Targets in Prostate Cancer Irish Association for Cancer Research [Details]|
|Toner, AP; Giles, FJ; Carleton, LA; O’Connell, E; Harte, E; Breen, L; Dunne. G; Clynes, M; Lewis, JD; Gorman, AM; Sullivan, FJ; Glynn, SA. (2014) Characterisation of a new class of prostate cancer chemotherapeutics Irish Association for Cancer Research [Details]|
|O'Reilly, P; O'Connell, E; Gernon, G; Szegezdi, E. (2014) Panel of 25 co-acting genes identified as a powerful predictor of TRAIL sensitivity Irish Association for Cancer Research [Details]|
|Cuffe, S; O’Connell, E; Morrison, C (2014) Inducing Centrosome Amplification to Radiosensitise Prostate Cancer Cells Irish Association for Cancer Research [Details]|
|Sandra Cuffe, Enda O'Connell, Ciaran Morrison (2013) Centrosome amplification as a strategy for radio-sensitisation of prostate cancer American Society for Cell Biology New Orleans, USA, , 14-DEC-13 - 18-DEC-13 [Details]|
|Toner, AP; Burke, A; Giles, FJ; Harte, E; Carleton, LA; O’Connell, E; Lewis, JD; Glynn, SA. (2013) Preclicincal analysis of EL102, a novel compound proposed for the treatment of prostate cancer Academic Screening Workshop Baltimore, MD, USA, [Details]|
|Toner, AP; McLaughlin, F; Giles, FJ; Sullivan, FJ; O’Connell, E; Carleton, LA; Breen, L; Gorman , AM; Lewis, JD and Glynn, SA. (2013) Introducing Novel Toluidine Sulphonamide EL102: A Potential Chemotherapeutic In Prostate Cancer British Association of Urological Surgeons AGM [Details]|
|Cuffe, S; Gaboriau, D; O’Connell, E; Morrison, C (2013) Centrosome amplification as a strategy for radiosensitization of prostate cancer Irish Association for Cancer Research [Details]|
|Murillo, L; Natoni, A; O’Connell, E; Fitzgerald, J; O’Dwyer, M; Santocanale, C (2013) Development of a High-Throughput Screening assay for the identification of compounds affecting Cdc7 dependent MCM helicase phosphorylation Irish Association for Cancer Research [Details]|
|Burke, AJ; O'Connell, E; Miranda, KM; Giles, FJ; Sullivan, F; Glynn, SA (2012) Nitric Oxide and its effects on cellular proliferation and phenotype in prostate cancer Royal Academy of Medicine in Ireland Annual Meeting NUI Galway, Ireland, [Details]|
|Monaghan, D; O’Connell, E; Fearnhead, HO. (2011) Repurposing the antifungal ciclopirox olamine for treatment of triple negative multi-drug resistant breast cancer Irish Association for Cancer Research Cork, [Details]|
|Flynn, A; Chen, XZ; O'Connell, E; Barry, F; O'Brien, T. (2011) The Pre-Conditioning of Unrestricted Somatic Stem Cells Alters Their Genotype, but Does Not Enhance Their Ability to Repair Cardiac Damage American College of Cardiology , pp.209-209 [Details]|
|Monaghan, D; Griffin, R; Regan, A; O’Connell, E; Fearnhead, H (2011) Repurposing Drugs For The Treatment of Multi-Drug Resistant Breast Cancer European Lab Automation Congress Hamburg, Germany, [Details]|
|Sharma, K; Talbot, A; Saleem, I; O'Connell, E; Rutherford, R; Gilmartin, JJ; O'Regan, A. (2010) Specific epithelial gene expression profile in acute sarcoidosis Irish Thoracic Society Cork, Ireland, , pp.492-492 [Details]|
|McCullagh K; Hughes O; Cooney R; O'Connell E; Mashayekhi K; Eglon M; O' Brien T. (2010) Gene Profile Analysis of NOS Transduced Human Arterial Smooth Muscle Cells Tissue Engineering and Regenerative Medicine International Society (TERMIS-EU) [Details]|
|Flynn, A,Chen, X,O'Connell, E,Duffy, A,Shaw, G,O'Loughlin, A,Dennedy, C,McHale, C,MacNeill, B,Nash, P,Crowley, J,Daly, K,Barry, F,O'Brien, T (2010) Unrestricted Somatic Stem Cells Improve Cardiac Function Post-Myocardial Infarction With and Without Pre-Conditioning. Authors Irish Cardiac Society AGM , pp.401-402 [Details]|
|Chandran, S; Mashayekhi, K; O'Connell, E; O'Brien, M; Smith, T. (2009) Estrogen Induced Differential Expression of Genes in Human Uterine Smooth Muscle Cells - A Global Analysis Society of Gynecological Investigation 56th Annual Meeting Glasgow, UK, , 17-MAR-09 - 21-MAR-09 , pp.112-113 [Details]|
|Byrne, GA; Russell, DA; O'Connell, EP; Meijer, WG. (2004) Transcriptional organisation and regulation of the vapA locus of the intracellular pathogen Rhodococcus equi Society for General Microbiology 155th Meeting Trinity College Dublin, Ireland, [Details]|
|Russell, DA; Byrne, GA; O'Connell, EP; Boland, CA; Meijer, WG. (2004) The LysR-Type Transcriptional Regulator VirR is Required for Expression of the Virulence Gene vapA of Rhodococcus equi ATCC 33701 Society for General Microbiology - Irish Branch NUI Galway, Ireland, [Details]|
|Duffy, P; O’Connell, E.; Meijer, W. (2002) Using Gene expression profiling to analyse the virulence of Rhodococcus equi Rhodococcus equi and Equine Rhodococcal Pneumonia Workshop Washington State University, Pullman, Washington, USA, [Details]|
| Employer: NUI Galway (NCBES)
Position: Senior Technical Officer (Functional Genomics and High Throughput Screening)
| Employer: NUI Galway (NCBES/Remedi/NBCRI)
Position: Microarray Technologist
| Year 2009 Institution: University College Dublin
Qualification: PhD Subject: Regulation of Virulence Gene Expression of the Intracellular Pathogen Rhodococcus equi.
| Year 2000 Institution: University College Dublin
Qualification: BSc Subject: Industrial Microbiology
| Year 1996 Institution: Garbally College Ballinasloe
Qualification: Leaving Certificate Subject: Various
Honours and Awards
| Year: 2012.
Title: "I'm a Scientist...get me out of here!" zone winner
| Year: 2014.
Title: Presidential Award for Volunteering
| Year: 2013.
Title: EXPLORE Innovation Initiative Award
| Year: 2008.
Title: Award in Team Leading
| Year: 2003.
Title: Open Postgraduate Scholarship Award
| Year: 2000.
Title: Research Scholarship
|ReelLIFE SCIENCE Schools Video Competition Director
|"I’m a Scientist, Get me out of Here", Ireland - Health Zone Winner 2012
|Association: Irish Science Teacher's Association (ISTA), Function/Role: Member|
|Enda is the Molecular Biology practical director for the MSc Biomedical Science programme http://ncbes.nuigalway.ie/msc-biomedical-science.aspx and MSc Biomedical Science via Distance Learning programme http://ncbes.nuigalway.ie/msc-biomedical-science-via-distance-learning.aspx.|