Prof Gerard Wall

BSc. PhD.

Contact Details

Personal Professor, Head of Microbiology
Microbiology

T: +353-91-495808
E: gerard.wall@nuigalway.ie
W: EU Consortium: https://sites.google.com/site/mariecurieiappconsortium/ Twitter: @jgerardwall
 

Biography

  • 2008-present: Senior Lecturer, Personal Professor; Microbiology, NUI Galway
  • 2015: Fulbright Scholar; Chemical Engineering, University of Wyoming, USA
  • 1996-2007:  Senior Lecturer, Lecturer, Assistant Lecturer; Industrial Biochemistry, University of Limerick
  • 1994-1996:  Postdoctoral Fellow (Molecular Immunology / Biochemistry); University of Zurich, Switzerland
  • 1990-1993:  PhD (Molecular Immunology); University of Aberdeen, Scotland
  • 1986-1990:  BSc 1st Hons. (Microbiology); NUI Galway
Research Interests:
  • (Bio)materials functionalisation; biomimetisation / functionalisation of materials - such as stents - using engineered recombinant proteins for addition- or modification-of-function.
  • Functional biomaterials in drug delivery: use of phage and peptide libraries to direct biodegradable nanospheres to specific cell types for targeted gene delivery
  • Expression of "difficult" proteins, such as glycosylated or glyco-engineered proteins and membrane proteins, in E. coli
  • Protein immobilisation studies: production and engineering (affinity maturation, increasing stability) of antibody fragments in E. coli for use in sensing, point-of-care, purification applications

Research Interests


Research Interests:
  • (Bio)materials functionalisation; biomimetisation / functionalisation of materials - such as stents - using engineered recombinant proteins for addition- or modification-of-function
  • Functional biomaterials in drug delivery: use of phage and peptide libraries to direct biodegradable nanospheres to specific cell types for targeted gene delivery
  • Expression of "difficult" proteins, such as glycosylated or glyco-engineered proteins and membrane proteins, in E. coli
  • Protein immobilisation studies: production and engineering (affinity maturation, increasing stability) of antibody fragments in E. coli for use in sensing, point-of-care, purification applications

Research Projects

  Project Start Date End Date
Development of a nebulised recombinant SOD protein for acute respiratory distress syndrome 01-JAN-17 31-DEC-17
CURAM Platform Gerard Wall 01-JUN-15 31-DEC-20
EPICSTENT GERARD WALL 01-APR-13 31-MAR-19

Contract Researchers

  Researcher Name Project Role Funding Body
Delphine Antoine Drug delivery in prostate cancer Postdoctoral FelLOW Other

Peer Reviewed Journals

  Year Publication
(2017) 'Battling bacterial infection with hexamethylene diisocyanate cross-linked and Cefaclor loaded collagen scaffolds'
Tsekoura E, Helling A, Wall JG, Bayon Y, Zeugolis D (2017) 'Battling bacterial infection with hexamethylene diisocyanate cross-linked and Cefaclor loaded collagen scaffolds'. Biomedical Materials, [Details]
(2016) 'Stainless steel surface functionalization for immobilization of antibody fragments for cardiovascular applications'
Foerster A, Holowacz I, Kumar GBS, Anandakumar S, Wall JG, Wawrzynska M, Paprocka M, Kantor A, Kraskiewicz H, Olsztynska-Janus S, Hinder SJ, Bialy D, Podbielska H, Kopaczynska M (2016) 'Stainless steel surface functionalization for immobilization of antibody fragments for cardiovascular applications'. Journal Of Biomedical Materials Research Part A, 104 :821-832 [DOI] [Details]
(2016) 'Adding Functions to Biomaterial Surfaces through Protein Incorporation'
Wronska, MA,O'Connor, IB,Tilbury, MA,Srivastava, A,Wall, JG (2016) 'Adding Functions to Biomaterial Surfaces through Protein Incorporation'. Advanced Materials, 28 :5485-5508 [DOI] [Details]
(2016) 'Poly (ethylene glycol) based hyper-branched polymer from RAFT and its application as a silver sulfadiazine loaded anti-bacterial hydrogel in wound care'
McMahon S, Kennedy R, Duffy P, Vasquez J, Wall JG, Tai H, Wang W (2016) 'Poly (ethylene glycol) based hyper-branched polymer from RAFT and its application as a silver sulfadiazine loaded anti-bacterial hydrogel in wound care'. Acs Applied Materials & Interfaces, 8 :26648-26656 [Details]
(2015) 'Improved Gene Transfection Efficacy and Cytocompatibility of Multifunctional Polyamidoamine-Cross-Linked Hyaluronan Particles'
Srivastava, A,Cunningham, C,Pandit, A,Wall, JG (2015) 'Improved Gene Transfection Efficacy and Cytocompatibility of Multifunctional Polyamidoamine-Cross-Linked Hyaluronan Particles'. Macromolecular Bioscience, 15 :682-690 [DOI] [Details]
(2014) 'Polymer-antibody fragment conjugates for biomedical applications'
Srivastava A, O'Connor IB, Pandit A, Wall JG (2014) 'Polymer-antibody fragment conjugates for biomedical applications'. Progress In Polymer Science, :308-329 [DOI] [Details]
(2013) 'Covalent and Oriented Immobilization of scFv Antibody Fragments via an Engineered Glycan Moiety'
Hu, XJ,Hortiguela, MJ,Robin, S,Lin, H,Li, YJ,Moran, AP,Wang, WX,Wall, JG (2013) 'Covalent and Oriented Immobilization of scFv Antibody Fragments via an Engineered Glycan Moiety'. Biomacromolecules, 14 :153-159 [DOI] [Details]
(2013) 'Thermal unfolding and refolding of lysozyme in deep eutectic solvents and their aqueous dilutions'
Esquembre, R,Sanz, JM,Wall, JG,del Monte, F,Mateo, CR,Ferrer, ML (2013) 'Thermal unfolding and refolding of lysozyme in deep eutectic solvents and their aqueous dilutions'. Physical Chemistry Chemical Physics, 15 :11248-11256 [DOI] [Details]
(2013) 'Isolation and Characterisation of a Recombinant Antibody Fragment That Binds NCAM1-Expressing Intervertebral Disc Cells'
Cunningham, C,Srivastava, A,Collin, E,Grad, S,Alini, M,Pandit, A,Wall, JG (2013) 'Isolation and Characterisation of a Recombinant Antibody Fragment That Binds NCAM1-Expressing Intervertebral Disc Cells'. Plos One, 8 [DOI] [Details]
(2013) 'Improved Detection of Domoic Acid Using Covalently Immobilised Antibody Fragments'
Hortiguela, MJ,Wall, JG (2013) 'Improved Detection of Domoic Acid Using Covalently Immobilised Antibody Fragments'. Marine Drugs, 11 :881-895 [DOI] [Details]
(2012) 'Interference: an alteRNAtive therapy following acute myocardial infarction'
Monaghan, M,Greiser, U,Wall, JG,O'Brien, T,Pandit, A (2012) 'Interference: an alteRNAtive therapy following acute myocardial infarction'. Trends In Pharmacological Sciences, 33 :635-645 [DOI] [Details]
(2011) 'Identification of cell surface-specific markers to target human nucleus pulposus cells: Expression of carbonic anhydrase XII varies with age and degeneration'
Power KA, Grad S, Rutges J, Creemers LB, van Rijen MHP, O'Gaora P, Wall JG, Alini M, Pandit A, Gallagher WM (2011) 'Identification of cell surface-specific markers to target human nucleus pulposus cells: Expression of carbonic anhydrase XII varies with age and degeneration'. Arthritis & Rheumatism , 63 :3876-3886 [DOI] [Details]
(2011) 'Antibody Immobilization on Solid Surfaces: Methods and Applications'
Hu, X,O'Connor, IB,Wall, JG,Tofail, SAM (2011) 'Antibody Immobilization on Solid Surfaces: Methods and Applications'. Biological Interactions With Surface Charge In Biomaterials, :90-104 [DOI] [Details]
(2010) 'Engineering of a fungal beta-galactosidase to remove product inhibition by galactose'
Hu XJ, Robin S, O'Connell S, Walsh G, Wall JG (2010) 'Engineering of a fungal beta-galactosidase to remove product inhibition by galactose'. Applied Microbiology And Biotechnology, 87 :1773-1782 [DOI] [Details]
(2009) 'Use of folding modulators to improve heterologous protein production in Escherichia coli'
Kolaj O, Spada S, Robin S, Wall JG (2009) 'Use of folding modulators to improve heterologous protein production in Escherichia coli'. Microbial Cell Factories, 8 [DOI] [Details]
(2009) 'Use of folding modulators to improve heterologous protein production in Escherichia coli'
Kolaj O, Spada S, Robin S, Wall JG (2009) 'Use of folding modulators to improve heterologous protein production in Escherichia coli'. Microbial Cell Factories, 8 [DOI] [Details]
(2009) 'Trigger Factor from the Psychrophilic Bacterium Psychrobacter frigidicola Is a Monomeric Chaperone'
Robin, S,Togashi, DM,Ryder, AG,Wall, JG (2009) 'Trigger Factor from the Psychrophilic Bacterium Psychrobacter frigidicola Is a Monomeric Chaperone'. Journal Of Bacteriology, 191 :1162-1168 [DOI] [Details]
(2009) 'Engineering of Cysteine Residues Leads to Improved Production of a Human Dipeptidase Enzyme in E. coli'
O'Dwyer, R,Razzaque, R,Hu, XJ,Hollingshead, SK,Wall, JG (2009) 'Engineering of Cysteine Residues Leads to Improved Production of a Human Dipeptidase Enzyme in E. coli'. Applied Biochemistry And Biotechnology, 159 :178-190 [DOI] [Details]
(2008) 'Insights into the mode of action of a putative zinc transporter CzrB in Thermus thermophilus'
V. Cherezov, N. Höfer, D.M.E. Szebenyi, O. Kolaj, J.G. Wall, R. Gillilan, V. Srinivasan, C.P. Jaroniec and M. Caffrey (2008) 'Insights into the mode of action of a putative zinc transporter CzrB in Thermus thermophilus'. Structure, 16 (9):1378-1388 [Details]
(2007) 'Crystallization and preliminary X-ray diffraction analysis of a soluble domain of the putative zinc transporter CzrB from Thermus thermophilus'
Höfer N, Kolaj O, Li H, Cherezov V, Gilliam R, Wall JG, Caffrey M (2007) 'Crystallization and preliminary X-ray diffraction analysis of a soluble domain of the putative zinc transporter CzrB from Thermus thermophilus'. ACTA CRYSTALLOGRAPHICA SECTION C-CRYSTAL STRUCTURE, 63 (8):673-677 [Details]
(2007) 'Optimisation of production of a domoic acid-binding scFv antibody fragment in Escherichia coli using molecular chaperones and functional immobilisation on a mesoporous silicate support'
Hu, XJ,O'Hara, L,White, S,Magner, E,Kane, M,Wall, JG (2007) 'Optimisation of production of a domoic acid-binding scFv antibody fragment in Escherichia coli using molecular chaperones and functional immobilisation on a mesoporous silicate support'. Protein Expression And Purification, 52 :194-201 [DOI] [Details]
(2006) 'The antibacterial effects of zinc ion migration from zinc based glass polyalkenoate cements'
Boyd D, Li H, Tanner DA, Chowdhury SA, Towler M, Wall JG (2006) 'The antibacterial effects of zinc ion migration from zinc based glass polyalkenoate cements'. JOURNAL OF MATERIALS SCIENCE: MATERIALS IN MEDICINE, 17 (6):489-494 [Details]
(2006) 'Adsorption and activity of a domoic acid-binding antibody fragment on mesoporous silicates'
Hu X, White S, Spada S, Hudson S, Magner E, Wall JG (2006) 'Adsorption and activity of a domoic acid-binding antibody fragment on mesoporous silicates'. JOURNAL OF PHYSICAL CHEMISTRY B, 110 (37):18703-18709 [Details]
(2005) 'Cloning, expression and characterisation of a single-chain Fv antibody fragment against domoic acid in Escherichia coli'
Hu X, O'Dwyer R, Wall JG (2005) 'Cloning, expression and characterisation of a single-chain Fv antibody fragment against domoic acid in Escherichia coli'. JOURNAL OF BIOTECHNOLOGY, 120 (1):38-45 [Details]
(2004) 'Measurement of the adsorption of cytochrome c onto the external surface of a thin film mesoporous silicate by ellipsometry'
Deere J, Serantoni M, Edler K, Hodnett BK, Wall JG, Magner E (2004) 'Measurement of the adsorption of cytochrome c onto the external surface of a thin film mesoporous silicate by ellipsometry'. LANGMUIR, 20 (2):532-536 [Details]
(2003) 'Oxidation of ABTS by silicate immobilized cytochrome c in nonaqueous solutions'
Deere J, Magner E, Wall JG, Hodnett BK (2003) 'Oxidation of ABTS by silicate immobilized cytochrome c in nonaqueous solutions'. BIOTECHNOLOGY PROGRESS, 19 (4):1238-1243 [Details]
(2003) 'A substrate variant as a high-affinity, reversible inhibitor: insight from the X-ray structure of cilastatin bound to membrane dipeptidase'
Smyth TP, Wall JG, Nitanai Y (2003) 'A substrate variant as a high-affinity, reversible inhibitor: insight from the X-ray structure of cilastatin bound to membrane dipeptidase'. Bioorg Med Chem, 11 (6):991-998 [Details]
(2003) 'Systemic lupus erythematosus in Portugal: Diagnosis and disease awareness from 1970-2001'
Moriarty T, O'Sullivan M, Tam A, Gonçalves RM, Wall JG (2003) 'Systemic lupus erythematosus in Portugal: Diagnosis and disease awareness from 1970-2001'. EUROPEAN JOURNAL OF EPIDEMIOLOGY, 18 (10):995-999 [Details]
(2003) 'Preliminary work on the antibacterial behaviour of strontium-containing glass ionomer cements'
Guida A, Towler MR, Wall JG, Hill RG, Eramo S (2003) 'Preliminary work on the antibacterial behaviour of strontium-containing glass ionomer cements'. 22 (20):1401-1403 [Details]
(2003) 'Adsorption and activity of proteins onto mesoporous silica'
Deere J, Magner E, Wall JG, Hodnett BK (2003) 'Adsorption and activity of proteins onto mesoporous silica'. CATALYSIS LETTERS, 85 (01-Feb):19-23 [Details]
(2002) 'Mechanistic and structural features of protein adsorption onto mesoporous silicates'
Deere J, Magner E, Wall JG, Hodnett BK (2002) 'Mechanistic and structural features of protein adsorption onto mesoporous silicates'. JOURNAL OF PHYSICAL CHEMISTRY B, 106 (29):7340-7347 [Details]
(2002) 'Isolation of a novel Thermus thermophilus metal efflux protein that improves E. coli growth under stress conditions'
Spada S, Pembroke JT, Wall JG (2002) 'Isolation of a novel Thermus thermophilus metal efflux protein that improves E. coli growth under stress conditions'. EXTREMOPHILES, 6 (4):301-308 [Details]
(2001) 'Cloning and characterisation of the hint homologue of the thermophile Thermus thermophilus'
Gibert Y, Spada S, Wall JG, Pembroke JT (2001) 'Cloning and characterisation of the hint homologue of the thermophile Thermus thermophilus'. DNA SEQUENCES, 12 (3):179-185 [Details]
(2001) 'Isolation and characterisation of the ylmE homologue of Thermus thermophilus'
Spada S, Gibert Y, Pembroke JT, Wall JG (2001) 'Isolation and characterisation of the ylmE homologue of Thermus thermophilus'. DNA SEQUENCES, 11 (6):507-514 [Details]
(2001) 'Adsorption and activity of cytochrome c adsorbed onto mesoporous silicates'
Deere J, Magner E, Wall JG, Hodnett BK (2001) 'Adsorption and activity of cytochrome c adsorbed onto mesoporous silicates'. CHEMICAL COMMUNICATIONS, 5 :466-465 [Details]
(2000) 'Lupus support and awareness in the Republic of Ireland'
Corcoran D, Wall JG (2000) 'Lupus support and awareness in the Republic of Ireland'. IRISH MEDICAL JOURNAL, 93 (2):54-55 [Details]
(1999) 'Clinical spectrum and diagnosis of systemic lupus erythematosus in the Republic of Ireland'
Corcoran D, O'Sullivan M, Wall JG (1999) 'Clinical spectrum and diagnosis of systemic lupus erythematosus in the Republic of Ireland'. MEDICAL SCIENCE RESEARCH, 27 (11):769-771 [Details]
(1999) 'The hierarchy of mutations influencing the folding of antibody domains in Escherichia coli'
Wall JG, Plückthun A (1999) 'The hierarchy of mutations influencing the folding of antibody domains in Escherichia coli'. PROTEIN ENGINEERING, 12 (7):605-611 [Details]
(1995) 'Effects of overexpressing folding modulators on the in vivo folding of heterologous proteins in Escherichia coli'
Wall JG, Plückthun A. (1995) 'Effects of overexpressing folding modulators on the in vivo folding of heterologous proteins in Escherichia coli'. Current Opinion in Biotechnology, 6 (5):507-516 [DOI] [Details]

Book Chapters

  Year Publication
(2017) 'Immobilisation of antibodies on cardiovascular stents'
O’Connor IB, Wall JG (2017) 'Immobilisation of antibodies on cardiovascular stents' In: Functionalized cardiovascular stents. Cambridge, MA, USA: Woodhead Publishing (Elsevier). [Details]
(2011) 'Antibody Immobilization on Solid Surfaces: Methods and Applications'
Hu X, O Connor IB, Wall JG (2011) 'Antibody Immobilization on Solid Surfaces: Methods and Applications' In: Biological Interactions with Surface Charge Biomaterials. London: Royal Society of Chemistry. [Details]
(1992) 'European Biotechnology Today Intercept Andover, U.K'
Wall JG, Cunningham C, Harris WJ (1992) 'European Biotechnology Today Intercept Andover, U.K' In: [Details]

Edited Books

  Year Publication
(2017) Functionalized cardiovascular stents.
Wall JG, Podbielska H, Wawrzyńska M (Ed.). (2017) Functionalized cardiovascular stents. Cambridge, MA, USA: Woodhead Publishing (Elsevier). [Details]

Conference Poster

  Year Publication
(2011) An IVD Regeneration Strategy using Targeted Gene Delivery. Poster presentation at the 24th European Conference on Biomaterials.
Cunningham, C., Grad, S., Alini, M., Pandit, A. and Wall, JG (2011) An IVD Regeneration Strategy using Targeted Gene Delivery. Poster presentation at the 24th European Conference on Biomaterials. Dublin: Conference Poster [Details]

Editorial

  Year Publication
(2018) Functionalized Cardiovascular Stents Preface.
Wall, JG,Podbielska, H,Wawrzynska, M (2018) Functionalized Cardiovascular Stents Preface. Editorial [Details]

Honours and Awards

  Year Title Awarding Body
2015 Fulbright Scholarship Fulbright

Professional Associations

  Association Function From / To
Irish Fulbright Alumni Association Member 01-JAN-16 /
Society for General Microbiology Ordinary member / Irish Section committee 01-JAN-00 /

Committees

  Committee Function From / To
Údarás na hOllscoile, NUIG Elected member 01-FEB-16 / 31-JAN-21
Standing and Strategic Planning Committee Member 01-FEB-16 /
Academic Planning and Resource Committee Member 01-FEB-16 / 31-JAN-21

Employment

  Employer Position From / To
University of Zurich, Switzerland Postdoctoral Fellow 01-JAN-94 / 31-AUG-96

Education

  Year Institution Qualification Subject
1994 University of Aberdeen, Scotland PhD Molecular Immunology
1990 NUI Galway BSc Microbiology

Teaching Interests

My broad teaching interests lie in the fields of molecular immunology, recombinant proteins. Current courses include:
  • Recombinant proteins: expression, engineering, exploitation (4th y BSc; MSc)
  • Workings of the immune system, immune interventions (3rd y BSc)
  • Medical microbiology, incl virology (2nd y BSc; 1st y BSc)

Recent Postgraduate Students

  Graduation Name Degree Primary Supervisor
2015 Malgorzata Wronska PhD Y
2015 Maura Tilbury Msc Y
2015 Claire Cunningham PhD Y

Current Postgraduate Students (Research)

  Student Degree Type Type
Maura Tilbury Doctorate - Structured PhD (Science) Supervisor

Modules Taught

  Term/Year Module Title Module Code Subject / Desc
Sem 2 Biology (Medical Microbiology) BO101 Human pathogens
Sem 2 Microbes and the Environment (Virology) MI204 Animal viruses, life cycle
Sem 2 Immunology and Recombinant Techniques MI324 Immune system, antibody engineering
Sem 1 Laboratory Project MI405 10 week lab-based research project for 4th y BSc students
Sem 2 Problem Solving Papers 1 and 2 MI413
Sem 2 Bioprocessors and Recombinant Protein Production MI442 RPP, scale up
Sem 2 Protein Technology BG5104 Rec protein expression, industrial scale up

Internal Collaborators

  Name Description of Collaboration
Professor Abhay Pandit, CÚRAM
Functional biomaterials / drug delivery: use of phage and peptide libraries to direct biodegradable nanospheres to specific cell types for targeted gene delivery; current applications in regeneration of degraded intervertebral disc regions (within the SFI-funded Centre for Research in Medical Devices - CÚRAM)
Dr Anne Marie Power
Identification, isolation, recombinant expression of marine-derived proteins of biomedical and biotechnological interest

External Collaborators

  Name Organisation / Institute Country Description of Collaboration
Professor Patrick Johnson University of Wyoming U.S.A.
Development of point of care diagnostic plaftforms
EU Epicstent Consortium Multiple
Functionalisation of cardiovascular stents for improved in vivo performance, carried out as part of an EU Consortium. Partners Balton Sp. z o.o., Wrocław Medical University, Wrocław University of Technology, Comenius University Bratislava.
Prof Francisco del Monte Group of Bioinspired Materials, ICCM, Madrid SPAIN
Investigation of novel (nano)supports for protein immobilisation, sensor develoment