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User Information

NSSLRL Request form (Salmonella, Shigella & Listeria)

NSSLRL User Guide

NSSLRL Accreditation Status

NSSLRL INAB Accreditation certificate

 

CPE Request form

If users require a service level agreement with the NSSLRL please download and return by post.

NSSLRL Service Level Agreement

Contact details

Head of Department

Prof.M.Cormican Tel: 353-(91) 524222

Laboratory

Tel: 353 (0)91 544628
Fax: 353 (0)91 495514
e-mail: niall.delappehse.ie ; jean.oconnorhse.ie

National Salmonella, Shigella & Listeria Reference Laboratory,
Department of Medical Microbiology,
University Hospital Galway,
Galway.

Services offered

Salmonella

• Serotyping and antimicrobial susceptibility testing  (AST)
• Phage typing of S.Enteritidis and S.Typhimurium
• Multi Locus Variable Analysis of S.Typhimurium

Salmonella Taxonomy

Shigella

• Serotyping and AST

Listeria 

• Biochemical identification and limited serotyping on clinical isolates or isolates from official food laboratories.

Molecular analysis (e.g. pulsed field gel electrophoresis) is performed when appropriate.

Ninety-five percent of samples will be reported within 15 days and specimens that are identified by telephone call as urgent will be prioritized. The average turnaround time is approximately 5 days.

The NSSLRL seeks to support research and development with respect to Salmonella, Shigella and Listeria in so far as resources permit. NSSLRL Research Co-operation policy

Opening hours

Mon – Fri 9:00am - 5:00pm (lunch from 1:00 – 2:00pm)

Out of hours work

This is performed at the discretion of the Consultant Microbiologist or the laboratory scientific staff on urgent samples, e.g. during an outbreak investigation.

Minimum requirements for completion of NSSLRL request forms

NSSLRL Request forms must be completed in legible handwriting or typing and all details must be entered. 

• Human isolates

The patient name and referring lab number/and or date of birth must be on both the form and side of slope.

If there is reason to suspect the isolate could be a Salmonella Typhi or Salmonella Paratyphi ,e.g. patient from an endemic country, this must be noted on the form.

If the isolate is suspected to be part of an outbreak this must be noted on the form.

• Non-human isolates

The referring lab number and isolate source must be on the form and side of slope.

Isolate source must be clearly stated, e.g. pork, chicken, beef, otherwise a report will not be issued.

Transportation of samples

Slopes must be packaged and transported according to IATA requirements.

Grow isolate overnight on a small nutrient agar slope and remove any fluid accumulating at the bottom of the slope using a sterile pasteur pipette.

Place slope(s) into an inner crushproof hard plastic container and place this into a cardboard box or envelope.

Label with an emergency contact number for the sender, and an Infectious substance label

Label box with UN3373 and add sticker with "BIOLOGICAL SUBSTANCE, CATEGORY B" .

This can be then sent to the NSSLRL via a courier company, e.g. Hays DX ( 01-8421088) , Capital Freight (01-8852064), Claymon (1800 252967) or Nightline Couriers (091-795100).

Discussion of reports

Please contact Prof. M.Cormican at martin.cormicanhse.ie , Dr. Deirbhile Keady at  deirbhile.keadyhse.ie, Dr. Una NiRiain at  una.niriainhse.ie, Dr.Teck Wee Boo at teck.boohse.ie .or Dr. Eithne McCarthy at eithne.mccarthyhse.ie. 

Note:  If users have a complaint or feel that any part of the service is unsatisfactory or could be improved in any way they should contact the laboratory.  

Complaints form

 

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