Biography

  • 2008-present:  Senior Lecturer, Microbiology, NUI Galway
  • 1996-2007:  Senior Lecturer, Lecturer, Assistant Lecturer, Industrial Biochemistry, University of Limerick
  • 1994-1996:  Postdoctoral Fellow (Molecular Immunology/Biochemistry), University of Zurich, Switzerland
  • 1990-1993:  PhD (Molecular Immunology), University of Aberdeen, Scotland
  • 1986-1990:  BSc 1st Hons. (Microbiology), NUI, Galway

Research Interests:
  • (Bio)materials functionalisation; biomimetisation / functionalisation of materials using engineered recombinant proteins for addition- or modification-of-function.
  • Functional biomaterials / drug delivery: use of phage and peptide libraries to direct biodegradable nanospheres to specific cell types for targeted gene delivery
  • Expression of "difficult" proteins, such as glycosylated or glyco-engineered proteins and membrane proteins, in E. coli
  • Protein immobilisation studies: production and engineering (affinity maturation, increasing stability) of antibody fragments in E. coli for use in sensing, purification applications
  • Research Interests

    Expression of recombinant proteins;
    Glycoprotein expression and engineering;
    Nanomedicine, targeted drug delivery;
    Protein immobilisation, sensor development;
    Materials biofunctionalisation.

    Research Projects

      Project Start Date End Date
    EPICSTENT GERARD WALL 01-APR-13 31-MAR-17
    EI CS PROG PROPOSAL PREPARATION SUPPORT FOR EU IAPP APPLICATION CS 2012 0498 GERARD WALL 01-FEB-12 26-SEP-13
    Expression and glycoengineering of glycoproteins in Escherichia coli 10-JAN-11 30-SEP-15
    Development of a targeted therapy for nucleus pulposus 01-SEP-08
    Trigger factor psychrobacter - g wall 01-JAN-09 31-DEC-09
    EI PC 2007 021 GERARD WALL 01-OCT-07 28-FEB-09
    BIO ELECTRIC SURFACE CPFP2125332 G WALL 01-OCT-08 01-NOV-12
    IRCSET EMPOWER POSTDOCTORAL FELLOWSHIP GERARD WALL DR MARIA HORTIGUELA 01-OCT-10 30-SEP-13

    Peer Reviewed Journals

      Year Publication
    (2014) 'Designing polymer-antibody fragment conjugates for biomedical applications'
    Srivastava A, O’Connor IB, Pandit A, Wall JG (2014) 'Designing polymer-antibody fragment conjugates for biomedical applications'. Progress In Polymer Science, 39 :308-329 [DOI] [Details]
    (2013) 'Thermal unfolding and refolding of lysozyme in deep eutectic solvents and their aqueous dilutions'
    Esquembre, R,Sanz, JM,Wall, JG,del Monte, F,Mateo, CR,Ferrer, ML (2013) 'Thermal unfolding and refolding of lysozyme in deep eutectic solvents and their aqueous dilutions'. Physical Chemistry Chemical Physics, 15 :11248-11256 [DOI] [Details]
    (2013) 'Improved detection of domoic Acid using covalently immobilised antibody fragments'
    Hortigüela MJ, Wall JG (2013) 'Improved detection of domoic Acid using covalently immobilised antibody fragments'. Marine Drugs, 11 (3):881-895 [DOI] [Details]
    (2013) 'Isolation and Characterisation of a Recombinant Antibody Fragment That Binds NCAM1-Expressing Intervertebral Disc Cells'
    Cunningham, C,Srivastava, A,Collin, E,Grad, S,Alini, M,Pandit, A,Wall, JG (2013) 'Isolation and Characterisation of a Recombinant Antibody Fragment That Binds NCAM1-Expressing Intervertebral Disc Cells'. Plos One, 8 [DOI] [Details]
    (2013) 'Covalent and oriented immobilization of scFv antibody fragments via an engineered glycan moiety'
    Hu X, Hortigüela MJ, Robin S, Lin H, Li Y, Moran AP, Wang W, Wall JG. (2013) 'Covalent and oriented immobilization of scFv antibody fragments via an engineered glycan moiety'. Biomacromolecules, 14 (1):153-159 [DOI] [Details]
    (2012) 'Interference: an alteRNAtive therapy following acute myocardial infarction'
    Monaghan M, Greiser U, Wall JG, O'Brien T, Pandit A (2012) 'Interference: an alteRNAtive therapy following acute myocardial infarction'. Trends In Pharmacological Sciences, [Details]
    (2011) 'Identification of cell surface-specific markers to target human nucleus pulposus cells: Expression of carbonic anhydrase XII varies with age and degeneration'
    Power KA, Grad S, Rutges J, Creemers LB, van Rijen MHP, O'Gaora P, Wall JG, Alini M, Pandit A, Gallagher WM (2011) 'Identification of cell surface-specific markers to target human nucleus pulposus cells: Expression of carbonic anhydrase XII varies with age and degeneration'. Arthritis & Rheumatism , 63 :3876-3886 [DOI] [Details]
    (2010) 'Engineering of a fungal beta-galactosidase to remove product inhibition by galactose'
    Hu XJ, Robin S, O'Connell S, Walsh G, Wall JG (2010) 'Engineering of a fungal beta-galactosidase to remove product inhibition by galactose'. Applied Microbiology And Biotechnology, 87 :1773-1782 [DOI] [Details]
    (2009) 'Use of folding modulators to improve heterologous protein production in Escherichia coli'
    Kolaj O, Spada S, Robin S, Wall JG (2009) 'Use of folding modulators to improve heterologous protein production in Escherichia coli'. Microbial Cell Factories, 8 [DOI] [Details]
    (2009) 'Engineering of cysteine residues leads to improved production of a human dipeptidase enzyme in E. Coli'
    O'Dwyer R, Razzaque R, Hu X, Hollingshead SK, Wall JG. (2009) 'Engineering of cysteine residues leads to improved production of a human dipeptidase enzyme in E. Coli'. Appl. Biochem. Biotechnol, 159 :178-190 [DOI] [Details]
    (2009) 'Trigger Factor from the Psychrophilic Bacterium Psychrobacter frigidicola Is a Monomeric Chaperone'
    Robin, S,Togashi, DM,Ryder, AG,Wall, JG (2009) 'Trigger Factor from the Psychrophilic Bacterium Psychrobacter frigidicola Is a Monomeric Chaperone'. Journal Of Bacteriology, 191 :1162-1168 [DOI] [Details]
    (2008) 'Insights into the mode of action of a putative zinc transporter CzrB in Thermus thermophilus'
    V. Cherezov, N. Höfer, D.M.E. Szebenyi, O. Kolaj, J.G. Wall, R. Gillilan, V. Srinivasan, C.P. Jaroniec and M. Caffrey (2008) 'Insights into the mode of action of a putative zinc transporter CzrB in Thermus thermophilus'. Structure, 16 (9):1378-1388 [Details]
    (2007) 'Crystallization and preliminary X-ray diffraction analysis of a soluble domain of the putative zinc transporter CzrB from Thermus thermophilus'
    Höfer N, Kolaj O, Li H, Cherezov V, Gilliam R, Wall JG, Caffrey M (2007) 'Crystallization and preliminary X-ray diffraction analysis of a soluble domain of the putative zinc transporter CzrB from Thermus thermophilus'. ACTA CRYSTALLOGRAPHICA SECTION C-CRYSTAL STRUCTURE, 63 (8):673-677 [Details]
    (2007) 'Optimisation of production of a domoic acid-binding scFv antibody fragment in Escherichia coli using molecular chaperones and functional immobilisation on a mesoporous silicate support'
    Hu, XJ,O'Hara, L,White, S,Magner, E,Kane, M,Wall, JG (2007) 'Optimisation of production of a domoic acid-binding scFv antibody fragment in Escherichia coli using molecular chaperones and functional immobilisation on a mesoporous silicate support'. Protein Expression And Purification, 52 :194-201 [DOI] [Details]
    (2006) 'Adsorption and activity of a domoic acid-binding antibody fragment on mesoporous silicates'
    Hu X, White S, Spada S, Hudson S, Magner E, Wall JG (2006) 'Adsorption and activity of a domoic acid-binding antibody fragment on mesoporous silicates'. JOURNAL OF PHYSICAL CHEMISTRY B, 110 (37):18703-18709 [Details]
    (2006) 'The antibacterial effects of zinc ion migration from zinc based glass polyalkenoate cements'
    Boyd D, Li H, Tanner DA, Chowdhury SA, Towler M, Wall JG (2006) 'The antibacterial effects of zinc ion migration from zinc based glass polyalkenoate cements'. JOURNAL OF MATERIALS SCIENCE: MATERIALS IN MEDICINE, 17 (6):489-494 [Details]
    (2005) 'Cloning, expression and characterisation of a single-chain Fv antibody fragment against domoic acid in Escherichia coli'
    Hu X, O'Dwyer R, Wall JG (2005) 'Cloning, expression and characterisation of a single-chain Fv antibody fragment against domoic acid in Escherichia coli'. JOURNAL OF BIOTECHNOLOGY, 120 (1):38-45 [Details]
    (2004) 'Measurement of the adsorption of cytochrome c onto the external surface of a thin film mesoporous silicate by ellipsometry'
    Deere J, Serantoni M, Edler K, Hodnett BK, Wall JG, Magner E (2004) 'Measurement of the adsorption of cytochrome c onto the external surface of a thin film mesoporous silicate by ellipsometry'. LANGMUIR, 20 (2):532-536 [Details]
    (2003) 'Preliminary work on the antibacterial behaviour of strontium-containing glass ionomer cements'
    Guida A, Towler MR, Wall JG, Hill RG, Eramo S (2003) 'Preliminary work on the antibacterial behaviour of strontium-containing glass ionomer cements'. 22 (20):1401-1403 [Details]
    (2003) 'Systemic lupus erythematosus in Portugal: Diagnosis and disease awareness from 1970-2001'
    Moriarty T, O'Sullivan M, Tam A, Gonçalves RM, Wall JG (2003) 'Systemic lupus erythematosus in Portugal: Diagnosis and disease awareness from 1970-2001'. EUROPEAN JOURNAL OF EPIDEMIOLOGY, 18 (10):995-999 [Details]
    (2003) 'Oxidation of ABTS by silicate immobilized cytochrome c in nonaqueous solutions'
    Deere J, Magner E, Wall JG, Hodnett BK (2003) 'Oxidation of ABTS by silicate immobilized cytochrome c in nonaqueous solutions'. BIOTECHNOLOGY PROGRESS, 19 (4):1238-1243 [Details]
    (2003) 'Adsorption and activity of proteins onto mesoporous silica'
    Deere J, Magner E, Wall JG, Hodnett BK (2003) 'Adsorption and activity of proteins onto mesoporous silica'. CATALYSIS LETTERS, 85 (01-Feb):19-23 [Details]
    (2003) 'A substrate variant as a high-affinity, reversible inhibitor: insight from the X-ray structure of cilastatin bound to membrane dipeptidase'
    Smyth TP, Wall JG, Nitanai Y (2003) 'A substrate variant as a high-affinity, reversible inhibitor: insight from the X-ray structure of cilastatin bound to membrane dipeptidase'. Bioorg Med Chem, 11 (6):991-998 [Details]
    (2002) 'Isolation of a novel Thermus thermophilus metal efflux protein that improves E. coli growth under stress conditions'
    Spada S, Pembroke JT, Wall JG (2002) 'Isolation of a novel Thermus thermophilus metal efflux protein that improves E. coli growth under stress conditions'. EXTREMOPHILES, 6 (4):301-308 [Details]
    (2002) 'Mechanistic and structural features of protein adsorption onto mesoporous silicates'
    Deere J, Magner E, Wall JG, Hodnett BK (2002) 'Mechanistic and structural features of protein adsorption onto mesoporous silicates'. JOURNAL OF PHYSICAL CHEMISTRY B, 106 (29):7340-7347 [Details]
    (2001) 'Adsorption and activity of cytochrome c adsorbed onto mesoporous silicates'
    Deere J, Magner E, Wall JG, Hodnett BK (2001) 'Adsorption and activity of cytochrome c adsorbed onto mesoporous silicates'. CHEMICAL COMMUNICATIONS, 5 :466-465 [Details]
    (2001) 'Isolation and characterisation of the ylmE homologue of Thermus thermophilus'
    Spada S, Gibert Y, Pembroke JT, Wall JG (2001) 'Isolation and characterisation of the ylmE homologue of Thermus thermophilus'. DNA SEQUENCES, 11 (6):507-514 [Details]
    (2001) 'Cloning and characterisation of the hint homologue of the thermophile Thermus thermophilus'
    Gibert Y, Spada S, Wall JG, Pembroke JT (2001) 'Cloning and characterisation of the hint homologue of the thermophile Thermus thermophilus'. DNA SEQUENCES, 12 (3):179-185 [Details]
    (2000) 'Lupus support and awareness in the Republic of Ireland'
    Corcoran D, Wall JG (2000) 'Lupus support and awareness in the Republic of Ireland'. IRISH MEDICAL JOURNAL, 93 (2):54-55 [Details]
    (1999) 'Clinical spectrum and diagnosis of systemic lupus erythematosus in the Republic of Ireland'
    Corcoran D, O'Sullivan M, Wall JG (1999) 'Clinical spectrum and diagnosis of systemic lupus erythematosus in the Republic of Ireland'. MEDICAL SCIENCE RESEARCH, 27 (11):769-771 [Details]
    (1999) 'The hierarchy of mutations influencing the folding of antibody domains in Escherichia coli'
    Wall JG, Plückthun A (1999) 'The hierarchy of mutations influencing the folding of antibody domains in Escherichia coli'. PROTEIN ENGINEERING, 12 (7):605-611 [Details]
    (1995) 'Effects of overexpressing folding modulators on the in vivo folding of heterologous proteins in Escherichia coli'
    Wall JG, Plückthun A. (1995) 'Effects of overexpressing folding modulators on the in vivo folding of heterologous proteins in Escherichia coli'. Current Opinion in Biotechnology, 6 (5):507-516 [DOI] [Details]

    Book Chapters

      Year Publication
    (2011) 'Antibody Immobilization on Solid Surfaces: Methods and Applications'
    Hu X, O Connor IB, Wall JG (2011) 'Antibody Immobilization on Solid Surfaces: Methods and Applications' In: Biological Interactions with Surface Charge Biomaterials. London: Royal Society of Chemistry. [Details]
    (1992) 'European Biotechnology Today Intercept Andover, U.K'
    Wall JG, Cunningham C, Harris WJ (1992) 'European Biotechnology Today Intercept Andover, U.K' In: [Details]

    Reviews

      Year Publication
    (2014) Polymer-antibody fragment conjugates for biomedical applications.
    Srivastava, A,O'Connor, LB,Pandit, A,Wall, JG (2014) Polymer-antibody fragment conjugates for biomedical applications. Reviews [DOI] [Details]
    (2012) Interference: an alteRNAtive therapy following acute myocardial infarction.
    Monaghan, M,Greiser, U,Wall, JG,O'Brien, T,Pandit, A (2012) Interference: an alteRNAtive therapy following acute myocardial infarction. Reviews [DOI] [Details]
    (2009) Use of folding modulators to improve heterologous protein production in Escherichia coli.
    Kolaj, O,Spada, S,Robin, S,Wall, JG (2009) Use of folding modulators to improve heterologous protein production in Escherichia coli. Reviews [DOI] [Details]

    Committees

      Committee Function From / To
    Society for General Microbiology 01-JAN-00 /

    Teaching Interests

  • Molecular immunology, recombinant proteins; courses include:
  • BO101, BO106 (1st y Science): Medical Microbiology
  • MI212 (2nd y Microbiology): Virology
  • MI317 (3rd y Microbiology): Immunology and Recombinant Techniques
  • MI442 (4th y Microbiology): Bioprocessors and Recombinant Protein Production
  • BG503 (MSc Biotechnology): Industrial Scale Protein Production